Reviewed, UniProtKB/Swiss-Prot A4YJR3 (RNPH_BRASO)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BRADO0173

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Ribonuclease PH HAMAP MF_00564		PRO_1000024779

Sequences

	Sequence		Length	Mass (Da)
	A4YJR3-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: E32EE9FC3816BA9A	FASTA	237	25,930
	10 20 30 40 50 60 MRPSRRAPDE MRAVSLERGV VKYAEGSCLV KFGDTHVLVT ATLEDRLPPW LKGQGRGWIT 70 80 90 100 110 120 AEYGMLPRAT LERTRREASA GKQTGRTVEI QRLIGRSLRT AIDLEALGER QITVDCDVLQ 130 140 150 160 170 180 ADGGTRTASI TGAWVALADC IRWMKARNMI KTEVLRTNVA AVSCGIYNGT PVLDLDYAED 190 200 210 220 230 SEAETDANFV MTGDGRIIEV QGTAEKTPFS EAEFLALMAL ARKGVGRLVD LQKMAVA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL74139.1.
RefSeq	YP_001202389.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YJR3.
Genome annotation databases
GeneID	5121098.
GenomeReviews	Gene locus BRADO0173 in contig CU234118_GR.
KEGG	bra:BRADO0173.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YAMLPRA.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BRASO

Accession

Primary (citable) accession number: A4YJR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)