Reviewed, UniProtKB/Swiss-Prot A4YJN0 (SYFA_BRASO)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BRADO0139

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000006803
Sites
	Metal binding	260	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YJN0-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: B907419F33008B05	FASTA	360	39,914
	10 20 30 40 50 60 MSDLASLETS ILDQIAAAGD EAALEAVRVA ALGKKGSISA LLATLGKMSP DERKTQGAAI 70 80 90 100 110 120 NLAKDKVTQA LSARRDVLKA AALDARLAAE TIDVTLPLQD TPADTGRIHP LSQVMDELTT 130 140 150 160 170 180 IFADMGFSIA EGPDVETDDY NFTKLNFPEG HPAREMHDTF FFNPKEDGSR MLLRTHTSPV 190 200 210 220 230 240 QVRTMLSQKP PIRVICPGRT YRIDSDATHT PQFHQVEGLV IDKGSHLGHL KWILHEFCKA 250 260 270 280 290 300 FFEVDHINMK FRPSFFPFTE PSLEVDIQCR RDKGEIRFGE GEDWLEILGC GMVHPNVLRA 310 320 330 340 350 360 CGIDPDEYQG FAWGMGIDRI AMLKYGMSDL RQLFEGDVRW LSHYGFKPLE VPTLAGGLST

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL74106.1.
RefSeq	YP_001202356.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YJN0.
Genome annotation databases
GeneID	5117746.
GenomeReviews	Gene locus BRADO0139 in contig CU234118_GR.
KEGG	bra:BRADO0139.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FRASYFP.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BRASO

Accession

Primary (citable) accession number: A4YJN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)