Reviewed, UniProtKB/Swiss-Prot A4YJJ3 (AROA_BRASO)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BRADO0098

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000012414

Sequences

	Sequence		Length	Mass (Da)
	A4YJJ3-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 67F5985124A30AC0	FASTA	445	46,189
	10 20 30 40 50 60 MSTSAAPTPL ESRASGPLSG TIRVPGDKSI SHRALILGAL SVGGTRISGL LEGEDVLNTA 70 80 90 100 110 120 KSMRTLGAKV ERTGEFAWTV NGVGVGGFAQ PAATLDFGNS GTGCRLVMGA VAGCPISAVF 130 140 150 160 170 180 DGDASLRSRP MRRILDPLAL MGAKVTASAE GGKLPLTLQG ASNPVPIEYR TPVASAQIKS 190 200 210 220 230 240 AVLLAGLAAP GVTTVIEQEA SRDHTELMLK HFGAEIVTTP EGSHGRRIAL TGQPELRGAP 250 260 270 280 290 300 VIVPADPSSA AFPLVAALIV DGSDLVLSDV MTNPLRTGLF TTLREMGASI EEDDVRGDAG 310 320 330 340 350 360 EPMARLRVRA SKLKGVEVPP ERAPSMIDEY LVLAVAAAYA EGTTIMRGLH ELRVKESDRL 370 380 390 400 410 420 EATAAMLRVN GVKVEITGDD LIVEGRGHVP GGGLVATHMD HRIAMSALVM GLASDKPVTV 430 440 DDTAFIATSF PDFIPLMRKA GADFA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL74069.1.
RefSeq	YP_001202319.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YJJ3.
Genome annotation databases
GeneID	5115933.
GenomeReviews	Gene locus BRADO0098 in contig CU234118_GR.
KEGG	bra:BRADO0098.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NPTRIEA.
Family and domain databases
HAMAP	MF_00210. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BRASO

Accession

Primary (citable) accession number: A4YJJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)