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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Metallosphaera sedula (strain ATCC 51363 / DSM 5348)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSED399549:GH1O-2165-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Msed_2131
OrganismiMetallosphaera sedula (strain ATCC 51363 / DSM 5348)
Taxonomic identifieri399549 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeMetallosphaera
ProteomesiUP000000242 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Glutamate--tRNA ligasePRO_1000071001Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi399549.Msed_2131.

Structurei

3D structure databases

ProteinModelPortaliA4YIL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi104 – 11411"HIGH" regionAdd
BLAST

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiGEYSGWD.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

A4YIL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMELEEIVY KYALWNAVKH NGQAQVGPVV SKVFAERPEL KANAKEVVKL
60 70 80 90 100
AEKMVAKVNA MSLEQQTAEL QKYPELLEER KKEEKKTLSP LPNVKGTVVT
110 120 130 140 150
RFAPNPDGPL HLGNARAAVL SFEYAKMYKG KFILRFDDTD PKVKKPIKEA
160 170 180 190 200
YDWIRDDLRW LNITWDLEFK ASERMSAYYN VAKVMLEKGF AYVDTLSDAE
210 220 230 240 250
FKAWRDSRNK TVYKPRTNPP EVNLELWEKM LNGDFDEGKA VVRIKTNPED
260 270 280 290 300
PDPSKIDWVM LRIIDTKRNP HPIAGDKFRV WPTYNFATAV DDHEFGITHI
310 320 330 340 350
LRAKEHTTNT EKQRWVYDYM GWEMPTVLEF GRLKLEGFMM SKSKIRGMLE
360 370 380 390 400
TGSERDDPRL PTLAGLRRRG IIPDTVREII IQVGLKVTDA TISFDNIASV
410 420 430 440 450
NRKLLDPVAK RLMFVREGVL FKLEIPQEMK AKVPLIPARQ EFREIFVKPG
460 470 480 490 500
DEIYLDKGDV EEGKVVRLMD LCNVKIEGDR LRFLSQDLES AKRMGANIIQ
510 520 530 540 550
WVKKSESKSV NVIKADPNKD VEEIRGYGEG YFETLKPGDI VQLVRYGFAR
560
VDSISRGEIT MIFAHE
Length:566
Mass (Da):65,210
Last modified:May 29, 2007 - v1
Checksum:i9121E10FC443A779
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000682 Genomic DNA. Translation: ABP96270.1.
RefSeqiWP_012022057.1. NC_009440.1.
YP_001192194.1. NC_009440.1.

Genome annotation databases

EnsemblBacteriaiABP96270; ABP96270; Msed_2131.
GeneIDi5104424.
KEGGimse:Msed_2131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000682 Genomic DNA. Translation: ABP96270.1.
RefSeqiWP_012022057.1. NC_009440.1.
YP_001192194.1. NC_009440.1.

3D structure databases

ProteinModelPortaliA4YIL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399549.Msed_2131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP96270; ABP96270; Msed_2131.
GeneIDi5104424.
KEGGimse:Msed_2131.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiGEYSGWD.

Enzyme and pathway databases

BioCyciMSED399549:GH1O-2165-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism."
    Auernik K.S., Maezato Y., Blum P.H., Kelly R.M.
    Appl. Environ. Microbiol. 74:682-692(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51363 / DSM 5348.

Entry informationi

Entry nameiSYE_METS5
AccessioniPrimary (citable) accession number: A4YIL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 29, 2007
Last modified: May 27, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.