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Protein

3-hydroxypropionyl-coenzyme A dehydratase

Gene

Msed_2001

Organism
Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in autotrophic carbon fixation via the 3-hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl-CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crotonyl-CoA. Inactive towards (R)-3-hydroxybutyryl-CoA.1 Publication

Catalytic activityi

3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O.1 Publication

Kineticsi

  1. KM=60 µM for 3-hydroxypropionyl-CoA1 Publication
  2. KM=75 µM for (S)-3-hydroxybutyryl-CoA1 Publication

    pH dependencei

    Optimum pH is 8.1 at room temperature. Retains half maximum activity at pH 6.5 and pH 9.5 at room temperature.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei113NucleophileBy similarity1
    Active sitei133Proton acceptorBy similarity1

    GO - Molecular functioni

    • 3-hydroxypropionyl-CoA dehydratase activity Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionLyase
    Biological processFatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13729
    BRENDAi4.2.1.116 7245

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-hydroxypropionyl-coenzyme A dehydratase1 Publication (EC:4.2.1.116)
    Short name:
    3-hydroxypropionyl-CoA dehydratase1 Publication
    Gene namesi
    Ordered Locus Names:Msed_2001
    OrganismiMetallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
    Taxonomic identifieri399549 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeMetallosphaera
    Proteomesi
    • UP000000242 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004046491 – 2593-hydroxypropionyl-coenzyme A dehydrataseAdd BLAST259

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi399549.Msed_2001

    Structurei

    3D structure databases

    ProteinModelPortaliA4YI89
    SMRiA4YI89
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the enoyl-CoA hydratase/isomerase family.Sequence analysis

    Phylogenomic databases

    eggNOGiENOG4102TIM Archaea
    COG1024 LUCA
    HOGENOMiHOG000027939
    KOiK15019
    OMAiCGIVARI
    OrthoDBiPOG093Z08GP

    Family and domain databases

    Gene3Di1.10.12.10, 1 hit
    InterProiView protein in InterPro
    IPR029045 ClpP/crotonase-like_dom_sf
    IPR018376 Enoyl-CoA_hyd/isom_CS
    IPR001753 Enoyl-CoA_hydra/iso
    IPR014748 enoyl-CoA_hydra_C
    PfamiView protein in Pfam
    PF00378 ECH_1, 1 hit
    SUPFAMiSSF52096 SSF52096, 1 hit
    PROSITEiView protein in PROSITE
    PS00166 ENOYL_COA_HYDRATASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    A4YI89-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEFETIETKK EGNLFWITLN RPDKLNALNA KLLEELDRAV SQAESDPEIR
    60 70 80 90 100
    VIIITGKGKA FCAGADITQF NQLTPAEAWK FSKKGREIMD KIEALSKPTI
    110 120 130 140 150
    AMINGYALGG GLELALACDI RIAAEEAQLG LPEINLGIYP GYGGTQRLTR
    160 170 180 190 200
    VIGKGRALEM MMTGDRIPGK DAEKYGLVNR VVPLANLEQE TRKLAEKIAK
    210 220 230 240 250
    KSPISLALIK EVVNRGLDSP LLSGLALESV GWGVVFSTED KKEGVSAFLE

    KREPTFKGK
    Length:259
    Mass (Da):28,316
    Last modified:May 29, 2007 - v1
    Checksum:i5B58E3747F1410BE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000682 Genomic DNA Translation: ABP96141.1
    RefSeqiWP_012021928.1, NC_009440.1

    Genome annotation databases

    EnsemblBacteriaiABP96141; ABP96141; Msed_2001
    GeneIDi5103388
    KEGGimse:Msed_2001

    Similar proteinsi

    Entry informationi

    Entry nameiHPCD_METS5
    AccessioniPrimary (citable) accession number: A4YI89
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 8, 2011
    Last sequence update: May 29, 2007
    Last modified: March 28, 2018
    This is version 65 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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