A4YI68 (PURL_METS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Msed_1980

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	702 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 702	702	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000072299
Regions
	Nucleotide binding	95 – 106	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A4YI68 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 18F3D83EBF6E3F1E	FASTA	702	75,625
	10 20 30 40 50 60 MILTLSKTEM EVVRRVLGRE PKEAEWKLVD ALWSEHCSYK SSKVFLRSLP STGPNVIMSV 70 80 90 100 110 120 EDWQDAGAVD VGDGLALVLK VESHNHPSAI DPFNGAATGV GGILRDIISK GAKPIALLDM 130 140 150 160 170 180 IRVGKLDDKG KWLLKNIISG IGFYGNSVGV PVVGGELSFD ETYNDNPLVD VACAGIVSKD 190 200 210 220 230 240 SIVPSVVREP GLRLVLAGFT GLDGLGGASF ASRKLSGMDE IGAVQIADPF AGKIIIDVTL 250 260 270 280 290 300 EIAKEVEAIK DLGGGGLAVA VTEMANGLGA VVNLERVPLR FEYLSPEDII ISETQERMLF 310 320 330 340 350 360 AVKPEKVESV CAKFREYNYP CEDIGEITAE PKVRFLYNGE PVAELPSDLL LSPPLNVWPA 370 380 390 400 410 420 ERKPRKREPE REVALGEALR AVLTHPDLVS KEWAYSQFDY EVGTSTVVKP GQGDSAVVEL 430 440 450 460 470 480 PNGKYLALKG DANQDLCAED AYECGKAIVA EAYRNLATVG ARGIALVDHL QFGDPRKPHV 490 500 510 520 530 540 YQDFIDAVRG ISEASKFFSI PVVGGKVSFH NEDKNGNPIK PTPLVVMAGL VEGKLARNRV 550 560 570 580 590 600 EEGDLVLIGE TRNELAGTLF SRIFGGGGEV AKTRLMEDLI ASNLVIKGIN EGKITWNKDV 610 620 630 640 650 660 SKGGLAGALL PILAKGFSVR ISSSQVIGTS NLLGKMFSES GGRFLVLTSD PQWFMYQAGR 670 680 690 700 MGIQALAIGK VTKDGSKLVL DYETFPMDSI VENYYSFLEG SL

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed: 18083856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP96120.1.
RefSeq	YP_001192044.1. NC_009440.1.
3D structure databases
ProteinModelPortal	A4YI68.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YI68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5103367.
GenomeReviews	Gene locus Msed_1980 in contig CP000682_GR.
KEGG	mse:Msed_1980.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MSED399549:MSED_1980-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METS5

Accession

Primary (citable) accession number: A4YI68

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program