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A4YI28 (SYL2_METS5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase 2

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase 2
Short name=LeuRS 2
Gene names
Name:leuS2
Ordered Locus Names:Msed_1940
OrganismMetallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]
Taxonomic identifier399549 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeMetallosphaera

Protein attributes

Sequence length950 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 950950Leucine--tRNA ligase 2 HAMAP-Rule MF_00049
PRO_0000334842

Regions

Motif47 – 5711"HIGH" region HAMAP-Rule MF_00049
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4YI28 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 3C6D2635D180537E

FASTA950109,016
        10         20         30         40         50         60 
MITIPSHEFT DLLNEISKKW QEEWSKNRIF EADPKDQKKF FTTVAFPYPN SPFHLGHGRT 

        70         80         90        100        110        120 
YVTGDVYARF MRMKGYNVLF PMGFHFTGTP IITMADDVAK GDKDLLDIFQ NIYEIPADVI 

       130        140        150        160        170        180 
PKLSDPLFMA NYFKEDIKAA MREIGLSIDW RREFTTIDPQ FSAFIVWQFS KLQKKGYVVK 

       190        200        210        220        230        240 
DTHPVGWCPV HNLPVGMHDT KGDMEPEIGE YVVIFFESKM GALAAATLRP ETIFGAVAVW 

       250        260        270        280        290        300 
VNPKATYTVA EIWGKKVIVS EKAAEKLKFQ TDVKVLEKVS GSDLLKIVAI NPITGKEIPI 

       310        320        330        340        350        360 
LPADFVDPTT ATGVVMSVPA HAPFDYFYLK KAKVGIEPIP VVAVEGQGDA PAKDLVESSH 

       370        380        390        400        410        420 
PKNDADLKKL TEQLYRLEFN KGLMRSDILR LVKDELRAEL SVVAGKQVPE ARKMVTDILI 

       430        440        450        460        470        480 
QRKAGTKMLE IMNKPVYCRC GNEVVVKILQ DQWFLDYGNP EWKAKAKKLL DSMRVIPEET 

       490        500        510        520        530        540 
RKDFEYALDW LQKRACARTR GLGTPLPWDK KWIIESLSDS TIYMAYYTLS HKIKEFGLHP 

       550        560        570        580        590        600 
SQLTEETWDY IMLGEGDVKA ISERNKIGVD ALQELRRHFT YWYPLDLRHS GPDLIPNHLS 

       610        620        630        640        650        660 
FFIFNHAGIF PENLWPRGVA VNGFILYEGK KMSKSLRNIV PLRKAIRTYG ADVIRIALSS 

       670        680        690        700        710        720 
LVDMSSDANF TEAGARAIAD NLKRFYELMQ MQDGSTIDGT PEKWLRSKLH RLVRDVTPLM 

       730        740        750        760        770        780 
ESMRFREVIN ELLFNLSSYI NEYLEMVRSE SREYNRDVIR EVVETWTKLM APFAPHLTEE 

       790        800        810        820        830        840 
MWHQLGHNTF LSLESWPTPD NSKINDQIEL EHEYHKLLIE DIRAILNVYK GKPSSVLLYV 

       850        860        870        880        890        900 
HDGSLNQVVK SALDVLNSGG TMKDFMQKNT PKSKEEARVL QRIMQYVTEM PETVKKLIYS 

       910        920        930        940        950 
NVNEMEVTRK GVPLLRYKLN LEIEVLAYTQ EVKQKLNKDA LPYRPAILVK 

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References

[1]"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism."
Auernik K.S., Maezato Y., Blum P.H., Kelly R.M.
Appl. Environ. Microbiol. 74:682-692(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000682 Genomic DNA. Translation: ABP96080.1.
RefSeqYP_001192004.2. NC_009440.1.

3D structure databases

ProteinModelPortalA4YI28.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399549.Msed_1940.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP96080; ABP96080; Msed_1940.
GeneID5103327.
KEGGmse:Msed_1940.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIDKWLLH.

Enzyme and pathway databases

BioCycMSED399549:GH1O-1971-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL2_METS5
AccessionPrimary (citable) accession number: A4YI28
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries