Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase 2

Gene

leuS2

Organism
Metallosphaera sedula (strain ATCC 51363 / DSM 5348)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei634 – 6341ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSED399549:GH1O-1971-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase 2UniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetase 2UniRule annotation
Short name:
LeuRS 2UniRule annotation
Gene namesi
Name:leuS2UniRule annotation
Ordered Locus Names:Msed_1940
OrganismiMetallosphaera sedula (strain ATCC 51363 / DSM 5348)
Taxonomic identifieri399549 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeMetallosphaera
ProteomesiUP000000242 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 950950Leucine--tRNA ligase 2PRO_0000334842Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi399549.Msed_1940.

Structurei

3D structure databases

ProteinModelPortaliA4YI28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi631 – 6355"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiFYITTCA.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

A4YI28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITIPSHEFT DLLNEISKKW QEEWSKNRIF EADPKDQKKF FTTVAFPYPN
60 70 80 90 100
SPFHLGHGRT YVTGDVYARF MRMKGYNVLF PMGFHFTGTP IITMADDVAK
110 120 130 140 150
GDKDLLDIFQ NIYEIPADVI PKLSDPLFMA NYFKEDIKAA MREIGLSIDW
160 170 180 190 200
RREFTTIDPQ FSAFIVWQFS KLQKKGYVVK DTHPVGWCPV HNLPVGMHDT
210 220 230 240 250
KGDMEPEIGE YVVIFFESKM GALAAATLRP ETIFGAVAVW VNPKATYTVA
260 270 280 290 300
EIWGKKVIVS EKAAEKLKFQ TDVKVLEKVS GSDLLKIVAI NPITGKEIPI
310 320 330 340 350
LPADFVDPTT ATGVVMSVPA HAPFDYFYLK KAKVGIEPIP VVAVEGQGDA
360 370 380 390 400
PAKDLVESSH PKNDADLKKL TEQLYRLEFN KGLMRSDILR LVKDELRAEL
410 420 430 440 450
SVVAGKQVPE ARKMVTDILI QRKAGTKMLE IMNKPVYCRC GNEVVVKILQ
460 470 480 490 500
DQWFLDYGNP EWKAKAKKLL DSMRVIPEET RKDFEYALDW LQKRACARTR
510 520 530 540 550
GLGTPLPWDK KWIIESLSDS TIYMAYYTLS HKIKEFGLHP SQLTEETWDY
560 570 580 590 600
IMLGEGDVKA ISERNKIGVD ALQELRRHFT YWYPLDLRHS GPDLIPNHLS
610 620 630 640 650
FFIFNHAGIF PENLWPRGVA VNGFILYEGK KMSKSLRNIV PLRKAIRTYG
660 670 680 690 700
ADVIRIALSS LVDMSSDANF TEAGARAIAD NLKRFYELMQ MQDGSTIDGT
710 720 730 740 750
PEKWLRSKLH RLVRDVTPLM ESMRFREVIN ELLFNLSSYI NEYLEMVRSE
760 770 780 790 800
SREYNRDVIR EVVETWTKLM APFAPHLTEE MWHQLGHNTF LSLESWPTPD
810 820 830 840 850
NSKINDQIEL EHEYHKLLIE DIRAILNVYK GKPSSVLLYV HDGSLNQVVK
860 870 880 890 900
SALDVLNSGG TMKDFMQKNT PKSKEEARVL QRIMQYVTEM PETVKKLIYS
910 920 930 940 950
NVNEMEVTRK GVPLLRYKLN LEIEVLAYTQ EVKQKLNKDA LPYRPAILVK
Length:950
Mass (Da):109,016
Last modified:May 29, 2007 - v1
Checksum:i3C6D2635D180537E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000682 Genomic DNA. Translation: ABP96080.1.
RefSeqiYP_001192004.2. NC_009440.1.

Genome annotation databases

EnsemblBacteriaiABP96080; ABP96080; Msed_1940.
GeneIDi5103327.
KEGGimse:Msed_1940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000682 Genomic DNA. Translation: ABP96080.1.
RefSeqiYP_001192004.2. NC_009440.1.

3D structure databases

ProteinModelPortaliA4YI28.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399549.Msed_1940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP96080; ABP96080; Msed_1940.
GeneIDi5103327.
KEGGimse:Msed_1940.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiFYITTCA.

Enzyme and pathway databases

BioCyciMSED399549:GH1O-1971-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism."
    Auernik K.S., Maezato Y., Blum P.H., Kelly R.M.
    Appl. Environ. Microbiol. 74:682-692(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51363 / DSM 5348.

Entry informationi

Entry nameiSYL2_METS5
AccessioniPrimary (citable) accession number: A4YI28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: July 22, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.