A4YI15 (ILVC_METS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Msed_1927

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000124312
Sites
	Active site	111	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A4YI15 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 10A165E9EEB411B9	FASTA	335	37,293
	10 20 30 40 50 60 MSHIAKIYTD KDTTLDPMKG KKIAVLGYGS QGRAWALNLR DSGLQVTVGL EREGKSWEQA 70 80 90 100 110 120 KADGFTPKKT EDAVKDADVV IFLVPDMAQR LVYRERVQPY LREGMDLVFA HGFNIHYRLI 130 140 150 160 170 180 EPPSNVDVYM VAPKGPGPIV RDFYVKGGGV PVLVAVHQNH SGKALEKALA IAKALGGTRA 190 200 210 220 230 240 GAIETTFKEE TETDLIGEQT ILVGGVMELM KAAFETLVEM GYQPEVAYFE TINELKMIVD 250 260 270 280 290 300 LIYDKGFMGM LRAVSDTAKY GGFTVGKQVI NEETRRRLRE AAEKVRSGKF AEEWIEEYGR 310 320 330 GSPTLSKGLE EMDKSLEEQT GRRLKEIIER GRPKS

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed: 18083856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP96067.1.
RefSeq	YP_001191991.1. NC_009440.1.
3D structure databases
ProteinModelPortal	A4YI15.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YI15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5103314.
GenomeReviews	Gene locus Msed_1927 in contig CP000682_GR.
KEGG	mse:Msed_1927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04335.
HOGENOM	HBG286525.
OMA	MAYAKGI.
PhylomeDB	A4YI15.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	MSED399549:MSED_1927-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METS5

Accession

Primary (citable) accession number: A4YI15

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 29, 2007
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program