A4YI09 (SYP_METS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Msed_1921

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Proline--tRNA ligase HAMAP MF_01571		PRO_1000073599

Sequences

	Sequence		Length	Mass (Da)
	A4YI09 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 7D3EE74E893E93EF	FASTA	480	54,960
	10 20 30 40 50 60 MKISREKWSS NFSEWFDWVI SQAEIYDYGR YPVKGSGVWM PYGFKIRQNV TTLIRKLLDE 70 80 90 100 110 120 TGHEEVLFPL LIPETLLKKE AEHIKGFEKE VFWVTHGGED ELEERFALRP TSEVAITLME 130 140 150 160 170 180 SLWIKGYSQL PRKFYQIVSV FRYETKATRP MIRVRELSTF KEAHTVHETF EDAARQVDEA 190 200 210 220 230 240 VEIYSKFFDI LGIPYLISRR PEWDKFAGAE YTIALDTIMP DGRALQIGTA HHLGQHFTKA 250 260 270 280 290 300 MDYKVQRADG SHVHPHQTSY GISDRVIATV ISINGDDHGP ILPPVVAPIE GVIIPIPGKS 310 320 330 340 350 360 EEDTEKINKY AMEVESVLKN SGIRVALDAS EDKTPGEKYY IWELKGVPIR IEIGPRELNS 370 380 390 400 410 420 GTAFLKRRDT LEGKSVKREE LVKEFRNLED QISADLRKRA WEQFKERVKR FQSLDEAKKF 430 440 450 460 470 480 LENRGGIAEV PWCGQDSCGL KIEEQVQARV LGTPLKPEPS GNCVVCGKPS TNILRIAKTY

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed: 18083856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP96061.1.
RefSeq	YP_001191985.1. NC_009440.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YI09.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5103308.
GenomeReviews	Gene locus Msed_1921 in contig CP000682_GR.
KEGG	mse:Msed_1921.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	A4YI09.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MSED399549:MSED_1921-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METS5

Accession

Primary (citable) accession number: A4YI09

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program