A4YI01 (SYM_METS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Msed_1913

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera › Metallosphaera sedula

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_1000075586
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	333 – 337	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YI01 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: D2C74532F099A8A1	FASTA	568	65,279
	10 20 30 40 50 60 MKILVASAWP YVQSVPHLGN LIGSILSADV FARYARLKYG KENVVFVSGS DEHGTPIEIE 70 80 90 100 110 120 AIKRGVSPKS LTDQAHEYDR QLFLNTWNIS FDNYTRTESE VHKSFVKEFL LGVSKYIKVE 130 140 150 160 170 180 EEELPYCERD KLFLPDRFVK GTCPYCGFED ARGDQCDRCG RLLTPSLLIN PKCAICGTPP 190 200 210 220 230 240 VLRKTKHWFF DLRPFSEPIR EWITSSQDMP ENVKGTALSW VNEGLKPRSL TRDNSWGIPA 250 260 270 280 290 300 PFPGAEGKTI YVWFEALLGY LSATLEYFQG RGETERWKEF WENGKVRSYY FIGKDNIPFH 310 320 330 340 350 360 AVILPAMLLA SEKNYALPTV IAATEYLMYE GQKFSKSRKI GIWIDEAPLI MEVDYWRFLL 370 380 390 400 410 420 IRMRPEEKDM NFTWTEAIRI VNSELNDDVG NLVNRVITMV NRYFQGKIPE PKNLKEVDMR 430 440 450 460 470 480 LLSRVGETLD QVSFMFEKGK LKGGTELVLT LARETNAYLN EKAPWDKVKS DVEDASNTLF 490 500 510 520 530 540 VASSAIRAIA LMLYPVIPEK AKLIYDQLGL DITQERWDNA KEPLKPGHVV GKPAPVFKKL 550 560 PQDFEKNLNE ILEKVRKEVE KRRPTLLK

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP96053.1.
RefSeq	YP_001191977.1. NC_009440.1.
3D structure databases
ProteinModelPortal	A4YI01.
ModBase	Search...
Protein-protein interaction databases
STRING	399549.Msed_1913.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABP96053; ABP96053; Msed_1913.
GeneID	5103300.
KEGG	mse:Msed_1913.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200402.
KO	K01874.
OMA	RMHGHEV.
ProtClustDB	PRK12268.
Enzyme and pathway databases
BioCyc	MSED399549:GH1O-1958-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_METS5

Accession

Primary (citable) accession number: A4YI01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program