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Protein

Ribonuclease Z

Gene

rnz

Organism
Metallosphaera sedula (strain ATCC 51363 / DSM 5348)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Zinc 1; catalyticUniRule annotation
Metal bindingi62 – 621Zinc 1; catalyticUniRule annotation
Active sitei64 – 641Proton acceptorUniRule annotation
Metal bindingi64 – 641Zinc 2; catalyticUniRule annotation
Metal bindingi65 – 651Zinc 2; catalyticUniRule annotation
Metal bindingi132 – 1321Zinc 1; catalyticUniRule annotation
Metal bindingi200 – 2001Zinc 1; catalyticUniRule annotation
Metal bindingi200 – 2001Zinc 2; catalyticUniRule annotation
Metal bindingi256 – 2561Zinc 2; catalyticUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMSED399549:GH1O-1816-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation)
Short name:
RNase ZUniRule annotation
Alternative name(s):
tRNA 3 endonucleaseUniRule annotation
tRNase ZUniRule annotation
Gene namesi
Name:rnzUniRule annotation
Ordered Locus Names:Msed_1788
OrganismiMetallosphaera sedula (strain ATCC 51363 / DSM 5348)
Taxonomic identifieri399549 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeMetallosphaera
Proteomesi
  • UP000000242 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Ribonuclease ZPRO_1000073684Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi399549.Msed_1788.

Structurei

3D structure databases

ProteinModelPortaliA4YHP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
PF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

A4YHP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKVYFIGTG GGAPSRRGLP AYLVRREGLS ILMDCGEGTQ ITMIRNSLNI
60 70 80 90 100
MNVNVIAITH LHADHVLGLP SLIQTMGMYD RKERLYILGP EGLKDLLTET
110 120 130 140 150
FERTYFSPNF PIEFVSSYES QGIRVRPFRT CHVVPSQGYL VEEKDTANLD
160 170 180 190 200
AERLRREGVT DWRVMRMLKE GKEVPWGDRV LKPEDYLIVK RGIRIAYTGD
210 220 230 240 250
TRPCETVINS VKGVDLLLHD STFEQGIDAS EYGHSTSTEA ATVAREAEVK
260 270 280 290
RLALIHISAR YRDTSEMLKQ ARRIFPMSFV PEDLSFLNLR A
Length:291
Mass (Da):33,031
Last modified:May 29, 2007 - v1
Checksum:i189B06379D5DF798
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000682 Genomic DNA. Translation: ABP95943.1.
RefSeqiWP_012021730.1. NC_009440.1.

Genome annotation databases

EnsemblBacteriaiABP95943; ABP95943; Msed_1788.
GeneIDi5104788.
KEGGimse:Msed_1788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000682 Genomic DNA. Translation: ABP95943.1.
RefSeqiWP_012021730.1. NC_009440.1.

3D structure databases

ProteinModelPortaliA4YHP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399549.Msed_1788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP95943; ABP95943; Msed_1788.
GeneIDi5104788.
KEGGimse:Msed_1788.

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Enzyme and pathway databases

BioCyciMSED399549:GH1O-1816-MONOMER.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
PF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism."
    Auernik K.S., Maezato Y., Blum P.H., Kelly R.M.
    Appl. Environ. Microbiol. 74:682-692(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51363 / DSM 5348.

Entry informationi

Entry nameiRNZ_METS5
AccessioniPrimary (citable) accession number: A4YHP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 29, 2007
Last modified: May 11, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.