Reviewed, UniProtKB/Swiss-Prot A4YHJ0 (RIFK_METS5)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Msed_1737

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Riboflavin kinase HAMAP MF_01285		PRO_0000322095
Regions
	Nucleotide binding	96 – 101	6	CDP By similarity
	Nucleotide binding	197 – 200	4	CDP By similarity
	Region	1 – 86	86	H-T-H motif-like HAMAP MF_01285
	Region	87 – 217	131	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	125	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Binding site	184	1	FMN By similarity
	Binding site	192	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YHJ0-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: C913F01E1E294DEB	FASTA	217	24,661
	10 20 30 40 50 60 MSDECLIAKI VYLSLQQKEV TQQMIADELK MSQQSVSRKL KELEERNLVR RSLSKEGEII 70 80 90 100 110 120 RVTENGEKLL EDCLSMMRAT IISSHVLEIR GRATSGLGEG RIFLSMPYYM ESFKKFLGFE 130 140 150 160 170 180 PYPGTLNLVI YDRISLENRL VLDISKAINI PEHKEENRVL GAVRAFPASV NDLKPAAVVF 190 200 210 PLRSVHPKSV IEVISPYHLR KELNIKDGDE VVIQAYA

References

[1]

"Complete sequence of Metallosphaera sedula DSM 5348." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Han C., Detter J.C., Bruce D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Maezato Y., Auernik K.S., Kelly R.M., Blum P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000682 Genomic DNA. Translation: ABP95892.1.
RefSeq	YP_001191816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YHJ0.
Genome annotation databases
GeneID	5105100.
GenomeReviews	Gene locus Msed_1737 in contig CP000682_GR.
KEGG	mse:Msed_1737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GAYYVRQ.
Family and domain databases
HAMAP	MF_01285. Fused. [Tree]
InterPro	IPR000835. HTH_MarR. IPR002834. Riboflavin_kinase_arc. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. PF01047. MarR. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_METS5

Accession

Primary (citable) accession number: A4YHJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)