A4YHJ0 (RIFK_METS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Msed_1737

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Riboflavin kinase HAMAP MF_01285		PRO_0000322095
Regions
	Nucleotide binding	96 – 101	6	CDP By similarity
	Nucleotide binding	197 – 200	4	CDP By similarity
	Region	1 – 86	86	H-T-H motif-like HAMAP MF_01285
	Region	87 – 217	131	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	125	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Binding site	184	1	FMN By similarity
	Binding site	192	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YHJ0 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: C913F01E1E294DEB	FASTA	217	24,661
	10 20 30 40 50 60 MSDECLIAKI VYLSLQQKEV TQQMIADELK MSQQSVSRKL KELEERNLVR RSLSKEGEII 70 80 90 100 110 120 RVTENGEKLL EDCLSMMRAT IISSHVLEIR GRATSGLGEG RIFLSMPYYM ESFKKFLGFE 130 140 150 160 170 180 PYPGTLNLVI YDRISLENRL VLDISKAINI PEHKEENRVL GAVRAFPASV NDLKPAAVVF 190 200 210 PLRSVHPKSV IEVISPYHLR KELNIKDGDE VVIQAYA

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed: 18083856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP95892.1.
RefSeq	YP_001191816.1. NC_009440.1.
3D structure databases
ProteinModelPortal	A4YHJ0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YHJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5105100.
GenomeReviews	Gene locus Msed_1737 in contig CP000682_GR.
KEGG	mse:Msed_1737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK802937.
Enzyme and pathway databases
BioCyc	MSED399549:MSED_1737-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_METS5

Accession

Primary (citable) accession number: A4YHJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program