Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Enolase

Gene

eno

Organism
Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (Msed_1412)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei152SubstrateUniRule annotation1
Binding sitei161SubstrateUniRule annotation1
Active sitei204Proton donorUniRule annotation1
Metal bindingi239MagnesiumUniRule annotation1
Metal bindingi282MagnesiumUniRule annotation1
Binding sitei282SubstrateUniRule annotation1
Metal bindingi308MagnesiumUniRule annotation1
Binding sitei308SubstrateUniRule annotation1
Active sitei333Proton acceptorUniRule annotation1
Binding sitei333Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei384SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:Msed_1668
OrganismiMetallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
Taxonomic identifieri399549 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeMetallosphaera
Proteomesi
  • UP000000242 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000720091 – 416EnolaseAdd BLAST416

Proteomic databases

PRIDEiA4YHC1

Interactioni

Protein-protein interaction databases

STRINGi399549.Msed_1668

Structurei

3D structure databases

ProteinModelPortaliA4YHC1
SMRiA4YHC1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni360 – 363Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169 Archaea
COG0148 LUCA
HOGENOMiHOG000072174
KOiK01689
OMAiEFMIIPV
OrthoDBiPOG093Z03D9

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

A4YHC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNGFSIANV QGYEIIDSRG NLTVRARVTL ESGIRATGDA PSGASKGTRE
60 70 80 90 100
AVELRDKDGS VKGAVDSINY YISPALMGLD VREQGKIDRI MIELDGTENK
110 120 130 140 150
SRLGANATIA TSIAVAKTAS ISMGLEPFMY IGGARTHTLP VPLLNILNGG
160 170 180 190 200
LHAGNMLKIQ EFMVIPVKFD TLKEALIAST KIYKTLKSLV TERYGKIYTA
210 220 230 240 250
LGDEGGISPP LSVTEDALKL VHEAIKRSGM EGRVFMGMDA AASDFYNPEK
260 270 280 290 300
GVYEIDNTSK SPDEMIEFYV DIASRYPLLY LEDPFEENDF SRYSELQSRI
310 320 330 340 350
KNVIVTGDDL FTTNVRYLRK GIEMKSARGV IVKANQIGTL TETIQFFDLA
360 370 380 390 400
KDNSIKTVVS HRSGETEDSF IADLAVGLNS DFIKTGAPSR GERTSKYNRL
410
LEIENEFGLE YLGRRL
Length:416
Mass (Da):45,751
Last modified:May 29, 2007 - v1
Checksum:iD8C0586EBEAF601F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000682 Genomic DNA Translation: ABP95823.1
RefSeqiWP_012021610.1, NC_009440.1

Genome annotation databases

EnsemblBacteriaiABP95823; ABP95823; Msed_1668
GeneIDi5105314
KEGGimse:Msed_1668

Similar proteinsi

Entry informationi

Entry nameiENO_METS5
AccessioniPrimary (citable) accession number: A4YHC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health