Reviewed, UniProtKB/Swiss-Prot A4YHA5 (G3P_METS5)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Msed_1652

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_1000072562
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	138 – 140	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	193 – 194	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	139	1	Nucleophile By similarity
	Binding site	109	1	NAD; via amide nitrogen By similarity
	Binding site	167	1	NAD By similarity
	Binding site	300	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YHA5-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 997600B78F9101A1	FASTA	340	36,648
	10 20 30 40 50 60 MIKVAVNGYG TIGKRVASAI LAQPDMTLVG VSKTSPNYEA YQAQKMGINL YVTKESLKEF 70 80 90 100 110 120 EEAGIKVKGV LEDMIKEADV VVDATPNGVG AQYKQVYSSM GKRALFQGGE KANVADVSFS 130 140 150 160 170 180 ALCNYDEAVD KRYVRVVSCN TTGLLRTLCT LNRVSKIAKV RGTIVRRAAD PKEVKKGPIN 190 200 210 220 230 240 SLVPDPASIP SHHAKDVNTV LKDLDIVTMA VVAPTTLMHV HLLDVTVSSP VTKEDVLSVL 250 260 270 280 290 300 SSTPRILLAS GKSKVSSTAE IVEVARDMGR PRNDLYELVV FEDSVSVRGN EVFLMYAVHQ 310 320 330 340 ESIVVPENVD AIRAVSGFAD GRKSIEMTNA SMGIGKGYLV

References

[1]

"Complete sequence of Metallosphaera sedula DSM 5348." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Han C., Detter J.C., Bruce D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Maezato Y., Auernik K.S., Kelly R.M., Blum P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000682 Genomic DNA. Translation: ABP95807.1.
RefSeq	YP_001191731.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5104857.
GenomeReviews	Gene locus Msed_1652 in contig CP000682_GR.
KEGG	mse:Msed_1652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4YHA5. AIFQGGE.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_METS5

Accession

Primary (citable) accession number: A4YHA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)