A4YEN9 (A4YEN9_METS5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Msed_0716

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. RuleBase RU004252 HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL ABP94891.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	41 – 48	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	43	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A4YEN9 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 73300C66FCD36863	FASTA	264	29,489
	10 20 30 40 50 60 MISNPTLKNY LDLDYGKVTD YLVNKLRDYI RDSGKKGGVV GVSGGIDSAV TATLLSRATQ 70 80 90 100 110 120 NFYFLIMPSN STPQQDLEDA LHLTEMLNGK DKRTIISIDN IVENFSNVVN TRDKLIVGNI 130 140 150 160 170 180 KARTRMILLY AFAQKMDYLV IGTGDKSELL LGYFTKYGDG GVDVLPIGDL FKTQVRKLGE 190 200 210 220 230 240 YLKLPSRIVS KPSSPALWEG QTAEGELGVS YEVVDPILYL LEKGKGDLEI TSELGVNLNL 250 260 VRRIRTMVER SRHKRLPPEI FKLP

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed: 18083856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP94891.1.
RefSeq	YP_001190815.1. NC_009440.1.
3D structure databases
ProteinModelPortal	A4YEN9.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YEN9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5103754.
GenomeReviews	Gene locus Msed_0716 in contig CP000682_GR.
KEGG	mse:Msed_0716.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4YEN9_METS5

Accession

Primary (citable) accession number: A4YEN9

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)