A4YEN4 (ILVD_METS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Msed_0711

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000321612
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A4YEN4 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: E763F9D757DABFF4	FASTA	557	59,177
	10 20 30 40 50 60 MYDKSRSNKV YGGYEKAPNR AFLKAMGLTD DDISKPLVGV AVAWNEAGPC NIHLLGLSQV 70 80 90 100 110 120 VKEGIRELGG TPRTFTAPVL IDGIAMGSES MKYSLVSREV IANTVELTVN GHGYDGFVAL 130 140 150 160 170 180 GGCDKTQPGL MMSMARLNIP SVYMYGGTTL PGNFRGRDIA IGDVYEAVGA FSAGKITAED 190 200 210 220 230 240 LRIMEDNAIP GPGACGGLYT ANTMAMLSEA LGLSLPGSSA PPAVSSDRTK FAKETGRTLM 250 260 270 280 290 300 KVMEIGLKPR DILTFEAFEN GIALLMASGG STNGVLHLLA IAHEAGVSLT LDDFDRISKK 310 320 330 340 350 360 VPEIVNMKPG GDYVMADLYR VGGTPVILKK LLDRGLLHGD TITVTGKTMA QNLSEYKIPE 370 380 390 400 410 420 FKHDHIVRDL SNPFLPSGGI RILKGSLAPE GSVVKLSASK IKYHRGPARV FNSEEEAFET 430 440 450 460 470 480 VLKKKINEGD VVVIRYEGPK GGPGMREMLA VTSAIVGQGL GEKVALVTDG RFSGATRGLM 490 500 510 520 530 540 VGHVAPEAAV GGPIALIRDG DTIVIDGEKG RLDVELSDQE LKSRAKDWTP PEPRYKTGLL 550 AQYAKLVTSS ARGAVLV

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed: 18083856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP94886.1.
RefSeq	YP_001190810.1. NC_009440.1.
3D structure databases
ProteinModelPortal	A4YEN4.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YEN4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5103749.
GenomeReviews	Gene locus Msed_0711 in contig CP000682_GR.
KEGG	mse:Msed_0711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04340.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	A4YEN4.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	MSED399549:MSED_0711-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. False negative. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_METS5

Accession

Primary (citable) accession number: A4YEN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	November 16, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program