A4YEC3 (RTCA_METS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Msed_0600

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000071720
Regions
	Nucleotide binding	279 – 282	4	ATP By similarity
Sites
	Active site	303	1	Tele-AMP-histidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YEC3 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 32AF906CB6A5C555	FASTA	334	36,271
	10 20 30 40 50 60 MLEIDGSFGE GGGQILRSSL ALSSLTGKGF RIRGIRAKRK NPGLQPQHLT SVRVMKMLTN 70 80 90 100 110 120 ARVSGDYLGS TELEFEPGEV VEGSFTVDVG TAGSVSLIAM TAIPLMINRR ISLRIRGGTD 130 140 150 160 170 180 VPLSPPVDYM RLVFIPLLEK MGITGKIEVK RRGHYPEGGG EVEVYGFRGE PRDLVLDSFG 190 200 210 220 230 240 ELREITGVSH VTSLPAHIAK RQIQGVMEVL REFNVPVKVE EEVSNEGSRG TGIVLSAHGL 250 260 270 280 290 300 SIMGASSLGE KGITAENVGR KAGTELLKEL KTGAAVDSHM GDMLVTFSAF ANLEYTGSLL 310 320 330 TQHTETNIEV IRKFLEIDVK IEGRSPFKLR LKRK

References

[1]

"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism." Auernik K.S., Maezato Y., Blum P.H., Kelly R.M. Appl. Environ. Microbiol. 74:682-692(2008) [PubMed: 18083856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000682 Genomic DNA. Translation: ABP94775.1.
RefSeq	YP_001190699.1. NC_009440.1.
3D structure databases
ProteinModelPortal	A4YEC3.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YEC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5105572.
GenomeReviews	Gene locus Msed_0600 in contig CP000682_GR.
KEGG	mse:Msed_0600.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04553.
HOGENOM	HBG683539.
OMA	GGTDVAW.
PhylomeDB	A4YEC3.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	MSED399549:MSED_0600-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF52913. RNA3'-term_phos_cycl_insert. 1 hit. SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_METS5

Accession

Primary (citable) accession number: A4YEC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program