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A4YE96 (SYL1_METS5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase 1

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase 1
Short name=LeuRS 1
Gene names
Name:leuS1
Ordered Locus Names:Msed_0573
OrganismMetallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]
Taxonomic identifier399549 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeMetallosphaera

Protein attributes

Sequence length938 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 938938Leucine--tRNA ligase 1 HAMAP-Rule MF_00049
PRO_0000334841

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif620 – 6245"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6231ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4YE96 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: FC5DCA901315932E

FASTA938107,432
        10         20         30         40         50         60 
MDSAFFNEVA KKWQEKWENN KVFEANPSNS EKYFITVAFP YTNSPLHIGH GRTYITADIV 

        70         80         90        100        110        120 
ARYQRMIGKN VLFPFAFQFT GTPILSISES IKRGDSDIIS DFINLYKISP EKVREFEDPL 

       130        140        150        160        170        180 
KLAEYFKEDM KRMAKALGLS VDWRREFTTI DPRFGQFIKW QFRKLKEKGF ITTATDAVGY 

       190        200        210        220        230        240 
CPNDNFPVGM HDTKGDVEPE VQEMDVIEFE GNDVVFPTAT SRPETVFGAN AVLINPEATY 

       250        260        270        280        290        300 
VLIRGSNWVL SKEAFRKLSY QRELVPEREV QGKDLIGLTV KNPISGKDVK VYGSKFVDAK 

       310        320        330        340        350        360 
MGTGSVMAVP AHEPLHYLGL SEVLSEVEVI PVISTEGYGD FPGPEVLALA GTKNPAELKD 

       370        380        390        400        410        420 
YIDTLYREEY YKGVMREDIV DLVPDYMRSI VKDRIAGKRV PEARRETVEL LRSLGKHDLI 

       430        440        450        460        470        480 
YEISNGPIYC RCGAEIVVKV IRDQWYITYD NPLWKSWTMK ALDRISIVPE EARRDMAKAI 

       490        500        510        520        530        540 
FSMKRRACSR SRGLGVKLPW DESQIIDSLS DSTIYTGFYT VAHKLSHDPS KLNDQFWDFV 

       550        560        570        580        590        600 
LLGNGDASEV SKVTGISVEE LKDLRNEFSY WYPLDSRHSG RDLVQNHLPF LIYNHLAIFG 

       610        620        630        640        650        660 
ESLLPRQIVI NGFVRVGGKK MSKSFRNIYP LYKAVEEYGV DPVRLALTIS SELLEDTDFD 

       670        680        690        700        710        720 
VNTVKAVTDQ LRRMYDLAVN LSKLRENEST GLPEKWLLSI IHYKVKEVSD LMNSLDLRKA 

       730        740        750        760        770        780 
FNIILYEYYE ILRDYLSMVS NPNTSVLRKA IEIWARLISP GAPHIAEEIW HIFNEGFVSL 

       790        800        810        820        830        840 
TRYPVPEELE VDGQAVIQLE YIRHLINQVK EISSMANKQP EKLIIYVSNS DELGILRAVL 

       850        860        870        880        890        900 
RGLKERKNLR ELSSITGQRE EYLRSLVERV QSLPPILREL IVTYPLDEFK TITDNLNFLV 

       910        920        930 
RRLDVDEIQV YRSDEANAPD IKGKKSNALP LLPGIVII 

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References

[1]"The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism."
Auernik K.S., Maezato Y., Blum P.H., Kelly R.M.
Appl. Environ. Microbiol. 74:682-692(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51363 / DSM 5348.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000682 Genomic DNA. Translation: ABP94748.1.
RefSeqYP_001190672.1. NC_009440.1.

3D structure databases

ProteinModelPortalA4YE96.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399549.Msed_0573.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP94748; ABP94748; Msed_0573.
GeneID5103733.
KEGGmse:Msed_0573.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAQFTGTPI.

Enzyme and pathway databases

BioCycMSED399549:GH1O-587-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL1_METS5
AccessionPrimary (citable) accession number: A4YE96
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries