Reviewed, UniProtKB/Swiss-Prot A4YD25 (DNLI_METS5)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Msed_0150

Organism

Metallosphaera sedula (strain ATCC 51363 / DSM 5348) [Complete proteome] [HAMAP]

Taxonomic identifier

399549 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Metallosphaera

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	DNA ligase		PRO_1000072271
Sites
	Active site	261	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YD25-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: A9C70DF05DA44BB0	FASTA	598	67,922
	10 20 30 40 50 60 MKFKLIAEYF DRLEKISSRI QLTSLLSDLF KNTEREVIDK VVYLIQGRLW PDFTGMPEIG 70 80 90 100 110 120 MGEKFLIKAI AMAYGNKEEE VEKLYKNIGD LGEVAYSLRS KVKGVSILSF VGGNQEAGEL 130 140 150 160 170 180 DVMEVYNELV KIATSTGEGS RDIKIRIFAG LIKKATPIEA KYLVRFVEGR LRLGIGDATV 190 200 210 220 230 240 LDALAITFGG SADYRPIVER AYNLRADLGD IARVIATEGI EKLKNISPTP GIPIRPMLAE 250 260 270 280 290 300 RLPDPEEIME KMNGKALVDY KYDGERAQIH RKGDKVTIFS RRMENITDQY IDVTEYVKQF 310 320 330 340 350 360 VKGDNFIVEG EIVPVDPESG EMRPFQELMH RRRKNNIAEA IKEYPVNLFL FDLMFFEGED 370 380 390 400 410 420 YTTKPLPERR AKLEEILASN DKVHIASHII ADRVDKLREY FYQAISEGAE GVMVKSIGPD 430 440 450 460 470 480 SIYQAGSRGW LWIKLKRDYQ SEMADTVDLV VVGAFYGKGK RGGKFSSLLM AAYNPEKDVF 490 500 510 520 530 540 ETVCKVASGF SDQELDEMQK KINELKREQK HPRVVSDMIP DVWVSPTLVA EVIGAEITIS 550 560 570 580 590 PLHTCCRGEK GGLSIRFPRF IRWRDDKSPE DATTNQEIME MYSKQLKKIE EKPVDENI

References

[1]

"Complete sequence of Metallosphaera sedula DSM 5348." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Han C., Detter J.C., Bruce D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Maezato Y., Auernik K.S., Kelly R.M., Blum P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000682 Genomic DNA. Translation: ABP94327.1.
RefSeq	YP_001190251.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5105003.
GenomeReviews	Gene locus Msed_0150 in contig CP000682_GR.
KEGG	mse:Msed_0150.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. [Graphical view]
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METS5

Accession

Primary (citable) accession number: A4YD25

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)