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Protein

Argininosuccinate synthase

Gene

argG

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei96 – 961CitrullineUniRule annotation
Binding sitei101 – 1011CitrullineUniRule annotation
Binding sitei126 – 1261ATP; via amide nitrogenUniRule annotation
Binding sitei128 – 1281AspartateUniRule annotation
Binding sitei132 – 1321AspartateUniRule annotation
Binding sitei132 – 1321CitrullineUniRule annotation
Binding sitei133 – 1331AspartateUniRule annotation
Binding sitei136 – 1361CitrullineUniRule annotation
Binding sitei185 – 1851CitrullineUniRule annotation
Binding sitei194 – 1941CitrullineUniRule annotation
Binding sitei270 – 2701CitrullineUniRule annotation
Binding sitei282 – 2821CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-3833-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Sputcn32_3714
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000006699 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Argininosuccinate synthasePRO_1000000434Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi319224.Sputcn32_3714.

Structurei

3D structure databases

ProteinModelPortaliA4YBT9.
SMRiA4YBT9. Positions 12-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4YBT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIENKNTGV KKVVLAYSGG LDTSAIIPWL KENYDNCEII AFCADVGQGE
60 70 80 90 100
EELVGLTEKA LASGASECHI VDLKEEFVKD YIYPTMATGA IYEGTYLLGT
110 120 130 140 150
SMARPIIAKA QVEVARKVGA DALCHGCTGK GNDQVRFEGC FAALAPDLKV
160 170 180 190 200
IAPWREWTMQ SREDLLDYLA ERNIKTSASA TKIYSRDANA FHISHEGGEL
210 220 230 240 250
EDPWNEPSKG VWTLTADPED APNQPEYVSL EVENGRVTKV NGEALTPYAA
260 270 280 290 300
LMKLNAIAAP HGVGRIDITE NRLVGMKSRG CYETPGGTVM FAALRAIEEL
310 320 330 340 350
VLDKTSRTWR EQVGAQMAHL VYDGRWFTPL CKSLLAASES LAESVNGEVV
360 370 380 390 400
VKLYKGHAIA VKKRSPNSLY SEAFATFGED QVYDQKHAEG FIRLYSLASR

IRALNAK
Length:407
Mass (Da):44,582
Last modified:May 29, 2007 - v1
Checksum:iDE3E33F711E184A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP77422.1.
RefSeqiWP_011920200.1. NC_009438.1.
YP_001185221.1. NC_009438.1.

Genome annotation databases

EnsemblBacteriaiABP77422; ABP77422; Sputcn32_3714.
KEGGispc:Sputcn32_3714.
PATRICi23556520. VBIShePut135485_3880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP77422.1.
RefSeqiWP_011920200.1. NC_009438.1.
YP_001185221.1. NC_009438.1.

3D structure databases

ProteinModelPortaliA4YBT9.
SMRiA4YBT9. Positions 12-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi319224.Sputcn32_3714.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP77422; ABP77422; Sputcn32_3714.
KEGGispc:Sputcn32_3714.
PATRICi23556520. VBIShePut135485_3880.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciSPUT319224:GHAP-3833-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CN-32 / ATCC BAA-453.

Entry informationi

Entry nameiASSY_SHEPC
AccessioniPrimary (citable) accession number: A4YBT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: April 29, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.