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A4YAA1 (A4YAA1_SHEPC) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172
Ordered Locus Names:Sputcn32_3172
OrganismShewanella putrefaciens (strain CN-32 / ATCC BAA-453) [Complete proteome] [HAMAP] EMBL ABP76884.1
Taxonomic identifier319224 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length756 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6421Zinc By similarity HAMAP MF_00172
Metal binding6441Zinc By similarity HAMAP MF_00172
Metal binding7271Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
A4YAA1 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 5E5951AF303C1EA5

FASTA75684,068
        10         20         30         40         50         60 
MQLNSLGFPR IGRRRELKFA LEKYWRGEST QAQLREVASE LRRTHWQWQA AAGIEQVPVG 

        70         80         90        100        110        120 
DFAFYDQVLT LSATLNAIPE RHRGEGAIDL DTLFRVARGR APTGQDAPAS EMTKYFNTNY 

       130        140        150        160        170        180 
HYLVPELSQN QEFSIAYEQF FDEVSEAQAL GYKAKPVLLG PVSYLFLAKT VGQEFDKLSL 

       190        200        210        220        230        240 
LPNLLTAYAD ILARFAAQGV QWVQLDEPIL ALELDGEWQA AITEAYQALS ITKVKILLTS 

       250        260        270        280        290        300 
YYGGIAHHQA LVSALPVAGL HVDLVTAPDQ LAVFANALGQ DQILSAGVVN GRNVWAAEAD 

       310        320        330        340        350        360 
LIAERIGTVA RDLGDRLWIG TSCSLLHSPV DLEVEATLES ALRQQLAFAK QKLLELANVR 

       370        380        390        400        410        420 
QLLLAPESVA AKEIVQTCLA RREAKAQAAD SQVIARVAAL TSANYERVSD FSVRQAVQQQ 

       430        440        450        460        470        480 
KYRLPLLPTT TIGSFPQTPA IRGLRSRWRK GELSDAQYTE QLQQVTRDTI DRQLKLGIDV 

       490        500        510        520        530        540 
LVHGEAERND MVEYFGEQLA GVGFTKNGWV QSYGSRCVKP PLIYGDVSRP KAMTVDWAEF 

       550        560        570        580        590        600 
AQSLTDKPVK GMLTGPVTIL HWSFAREDIG RDVIATQLAL AIRDEVVDLQ NAGIGIIQID 

       610        620        630        640        650        660 
EPAFREGLPL KQSEWQAYLD WAVNAFKLSA AGVIDETQIH THMCYSEFND TIAAIAAMDA 

       670        680        690        700        710        720 
DVITIETSRS RMELLNAFED FEYPNEIGPG VYDIHSPNTP SVEAMVHLIE KAAQKVPVRQ 

       730        740        750 
LWVNPDCGLK TRTWDEVEPA LKNMVDATRE LRRRLG

« Hide

References

[1]"Complete sequence of Shewanella putrefaciens CN-32."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Fredrickson J., Tiedje J., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CN-32 / ATCC BAA-453.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000681 Genomic DNA. Translation: ABP76884.1.
RefSeqYP_001184683.1. NC_009438.1.

3D structure databases

ProteinModelPortalA4YAA1.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4YAA1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5080115.
GenomeReviewsGene locus Sputcn32_3172 in contig CP000681_GR.
KEGGspc:Sputcn32_3172.
NMPDRfig|319224.13.peg.2925.
PATRIC23555378. VBIShePut135485_3322.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0620.
HOGENOMHBG287495.
OMAMLDTAVM.
PhylomeDBA4YAA1.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA4YAA1_SHEPC
AccessionPrimary (citable) accession number: A4YAA1
Entry history
Integrated into UniProtKB/TrEMBL: May 29, 2007
Last sequence update: May 29, 2007
Last modified: December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info