Reviewed, UniProtKB/Swiss-Prot A4Y9Z3 (CYSI_SHEPC)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	Sputcn32_3063

Organism

Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) [Complete proteome] [HAMAP]

Taxonomic identifier

319224 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_1000068770
Sites
	Metal binding	429	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	435	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	474	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	478	1	Iron (siroheme axial ligand) By similarity
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4Y9Z3-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 9EB54A8EA6000927	FASTA	565	62,996
	10 20 30 40 50 60 MSEQKLALNE YLKTDSDYLR GTIKEGLDSS VTGSFSDGDQ QLIKFHGFYQ QDDRDLRNER 70 80 90 100 110 120 KEQKLEPLYS FMLRARVPGG ICSPQQWLGV DKIASTLTSS NSIRLTTRQT FQYHGIPKRN 130 140 150 160 170 180 LKTIIQDLDR QALDSIAACG DVNRNVMCNP NPVESKLHEQ AYAVAKKLSD HLLPHTRAYA 190 200 210 220 230 240 EIWLDEEKLL TTEDETVEPV YGKTYLPRKF KMAVAVPPDN DVDVYTNDLG FIAVAENGEL 250 260 270 280 290 300 VGFNLTAGGG MGSTHGEVET FPRLADDFGF IKTEDVMKFA EAVMTVQRDW GNRSNRKRSR 310 320 330 340 350 360 LKYTIVDHGY EKFKAEVEAR AGVKFEPKRE VVIGDRGDRY GWVEGVDGKW HLTLFIESGR 370 380 390 400 410 420 IKDLPGQTLQ TGLREIAKIH KGDFRMTSNQ NMIIAGVAAE DKATIEGLAR KHGLLGQVLT 430 440 450 460 470 480 QTRGHSIACV ALPTCPLAMA EAERYFPEFI DHIDALQAKH GISEQAIVVR MTGCPNGCAR 490 500 510 520 530 540 PFAAEIGLVG KAPGRYNLYL GASFEGTRLN KMHRENIQEA DILAELDTLF GRYAVERDAG 550 560 ETFGNFTVRV GVVKAVIDAA KDFHG

References

[1]

"Complete sequence of Shewanella putrefaciens CN-32." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Fredrickson J., Tiedje J., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000681 Genomic DNA. Translation: ABP76776.1.
RefSeq	YP_001184575.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5079703.
GenomeReviews	Gene locus Sputcn32_3063 in contig CP000681_GR.
KEGG	spc:Sputcn32_3063.
NMPDR	fig|319224.13.peg.2821.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_SHEPC

Accession

Primary (citable) accession number: A4Y9Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)