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A4Y9G2

- LIPA_SHEPC

UniProt

A4Y9G2 - LIPA_SHEPC

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Protein

Lipoyl synthase

Gene
lipA, Sputcn32_2876
Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-2980-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Sputcn32_2876
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000006699: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Lipoyl synthaseUniRule annotationPRO_1000012277Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi319224.Sputcn32_2876.

Structurei

3D structure databases

ProteinModelPortaliA4Y9G2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A4Y9G2-1 [UniParc]FASTAAdd to Basket

« Hide

MNRPERLQPG VKLRDADKVS RIPVKIVPSE RDTMLRKPDW LRVKLPASNQ    50
RILDIKQALR SNGLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF 100
CDVAHGRPLK PDAEEPVKLA QTIRDMKLKY VVITSVDRDD LRDGGAQHFA 150
DCIREIRKLN PAIKIEILVP DFRGRIDAAL DILATEPPDV FNHNLETAPM 200
HYRKARPGAN YQWSLDLLKR FKERHPNVPT KSGLMMGLGE TNEEIAQVLR 250
DLREHKVEML TLGQYLQPSK FHLPVERYVP PAEFDELKAL ADELGFTHAA 300
CGPLVRSSYH ADLQAQGKEV K 321
Length:321
Mass (Da):36,345
Last modified:May 29, 2007 - v1
Checksum:i13E3D19B159ECCF4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000681 Genomic DNA. Translation: ABP76595.1.
RefSeqiYP_001184394.1. NC_009438.1.

Genome annotation databases

EnsemblBacteriaiABP76595; ABP76595; Sputcn32_2876.
GeneIDi5078623.
KEGGispc:Sputcn32_2876.
PATRICi23554744. VBIShePut135485_3007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000681 Genomic DNA. Translation: ABP76595.1 .
RefSeqi YP_001184394.1. NC_009438.1.

3D structure databases

ProteinModelPortali A4Y9G2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 319224.Sputcn32_2876.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP76595 ; ABP76595 ; Sputcn32_2876 .
GeneIDi 5078623.
KEGGi spc:Sputcn32_2876.
PATRICi 23554744. VBIShePut135485_3007.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci SPUT319224:GHAP-2980-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CN-32 / ATCC BAA-453.

Entry informationi

Entry nameiLIPA_SHEPC
AccessioniPrimary (citable) accession number: A4Y9G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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