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A4Y9B5

- PNP_SHEPC

UniProt

A4Y9B5 - PNP_SHEPC

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, Sputcn32_2829
Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851Magnesium By similarity
Metal bindingi491 – 4911Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-2924-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Sputcn32_2829
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000006699: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 699699Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329843Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi319224.Sputcn32_2829.

Structurei

3D structure databases

ProteinModelPortaliA4Y9B5.
SMRiA4Y9B5. Positions 616-691.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61160KH
Add
BLAST
Domaini621 – 68969S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4Y9B5-1 [UniParc]FASTAAdd to Basket

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MNPIVKSFEY GQHTVTLETG VIARQADAAV LASMGDTTVL VTVVGKKEAE    50
AGRDFFPLTV NYQEKTYAAG KIPGGFFKRE GRPSEDETLI ARLIDRPIRP 100
LFPNGFKNEV QVIITVVSVD PQIEPDIISM IGTSAALAIS GIPFSGPLGA 150
ARVGYINGEY VLNPTVAQIE TSQLNLVVAG TASAVLMVES EAQALPEEVM 200
LGSVVYGHDQ QQVVINAIAE FKAEAGKPMW DWTAPVQDET LVAQIKELAE 250
AGLGDAYKIQ VKQDRYAQVA VVKAATKEAL LASNPNIDLR EVDNLLGSLE 300
KKVVRGRIIR GEPRIDGREP DMIRALSVLA GVLPRTHGSA LFTRGETQAL 350
VTCTLGTERD AQKIDSIMGE RTNRFMLHYN FPPYSVGETG MVGSPKRREI 400
GHGKLAWRGI NAVMPSAEEF PYSVRVVSEI TESNGSSSMA SVCGTSLALM 450
DAGVPIKTSV AGIAMGLVKE GDDFVVLSDI LGDEDHLGDM DFKVAGTRGG 500
ITALQMDIKI EGITKEIMEI ALQQAYGARV HILNVMDQAI GSHRDDISDH 550
APRITTIKIN PEKIRDVIGK GGAVIRALTE ETGTTIELED DGTVRIASSN 600
GEATKEAIRR IEEITSEVEV GRIYNGKVIR IVDFGAFVNI LPGKDGLVHI 650
SQISDERVAN VSDHLELNQE VAVKVMEVDR QGRVRLSIKE AQTKEAAAE 699
Length:699
Mass (Da):75,453
Last modified:May 29, 2007 - v1
Checksum:i84ACB5302ECC4249
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000681 Genomic DNA. Translation: ABP76548.1.
RefSeqiYP_001184347.1. NC_009438.1.

Genome annotation databases

EnsemblBacteriaiABP76548; ABP76548; Sputcn32_2829.
GeneIDi5078661.
KEGGispc:Sputcn32_2829.
PATRICi23554626. VBIShePut135485_2957.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000681 Genomic DNA. Translation: ABP76548.1 .
RefSeqi YP_001184347.1. NC_009438.1.

3D structure databases

ProteinModelPortali A4Y9B5.
SMRi A4Y9B5. Positions 616-691.
ModBasei Search...

Protein-protein interaction databases

STRINGi 319224.Sputcn32_2829.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP76548 ; ABP76548 ; Sputcn32_2829 .
GeneIDi 5078661.
KEGGi spc:Sputcn32_2829.
PATRICi 23554626. VBIShePut135485_2957.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci SPUT319224:GHAP-2924-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CN-32 / ATCC BAA-453.

Entry informationi

Entry nameiPNP_SHEPC
AccessioniPrimary (citable) accession number: A4Y9B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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