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A4Y9B5

- PNP_SHEPC

UniProt

A4Y9B5 - PNP_SHEPC

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi485 – 4851MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciSPUT319224:GHAP-2924-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Sputcn32_2829
    OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
    Taxonomic identifieri319224 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000006699: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 699699Polyribonucleotide nucleotidyltransferasePRO_0000329843Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Protein-protein interaction databases

    STRINGi319224.Sputcn32_2829.

    Structurei

    3D structure databases

    ProteinModelPortaliA4Y9B5.
    SMRiA4Y9B5. Positions 616-691.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini552 – 61160KHUniRule annotationAdd
    BLAST
    Domaini621 – 68969S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4Y9B5-1 [UniParc]FASTAAdd to Basket

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    MNPIVKSFEY GQHTVTLETG VIARQADAAV LASMGDTTVL VTVVGKKEAE    50
    AGRDFFPLTV NYQEKTYAAG KIPGGFFKRE GRPSEDETLI ARLIDRPIRP 100
    LFPNGFKNEV QVIITVVSVD PQIEPDIISM IGTSAALAIS GIPFSGPLGA 150
    ARVGYINGEY VLNPTVAQIE TSQLNLVVAG TASAVLMVES EAQALPEEVM 200
    LGSVVYGHDQ QQVVINAIAE FKAEAGKPMW DWTAPVQDET LVAQIKELAE 250
    AGLGDAYKIQ VKQDRYAQVA VVKAATKEAL LASNPNIDLR EVDNLLGSLE 300
    KKVVRGRIIR GEPRIDGREP DMIRALSVLA GVLPRTHGSA LFTRGETQAL 350
    VTCTLGTERD AQKIDSIMGE RTNRFMLHYN FPPYSVGETG MVGSPKRREI 400
    GHGKLAWRGI NAVMPSAEEF PYSVRVVSEI TESNGSSSMA SVCGTSLALM 450
    DAGVPIKTSV AGIAMGLVKE GDDFVVLSDI LGDEDHLGDM DFKVAGTRGG 500
    ITALQMDIKI EGITKEIMEI ALQQAYGARV HILNVMDQAI GSHRDDISDH 550
    APRITTIKIN PEKIRDVIGK GGAVIRALTE ETGTTIELED DGTVRIASSN 600
    GEATKEAIRR IEEITSEVEV GRIYNGKVIR IVDFGAFVNI LPGKDGLVHI 650
    SQISDERVAN VSDHLELNQE VAVKVMEVDR QGRVRLSIKE AQTKEAAAE 699
    Length:699
    Mass (Da):75,453
    Last modified:May 29, 2007 - v1
    Checksum:i84ACB5302ECC4249
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP76548.1.
    RefSeqiYP_001184347.1. NC_009438.1.

    Genome annotation databases

    EnsemblBacteriaiABP76548; ABP76548; Sputcn32_2829.
    GeneIDi5078661.
    KEGGispc:Sputcn32_2829.
    PATRICi23554626. VBIShePut135485_2957.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP76548.1 .
    RefSeqi YP_001184347.1. NC_009438.1.

    3D structure databases

    ProteinModelPortali A4Y9B5.
    SMRi A4Y9B5. Positions 616-691.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 319224.Sputcn32_2829.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP76548 ; ABP76548 ; Sputcn32_2829 .
    GeneIDi 5078661.
    KEGGi spc:Sputcn32_2829.
    PATRICi 23554626. VBIShePut135485_2957.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci SPUT319224:GHAP-2924-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CN-32 / ATCC BAA-453.

    Entry informationi

    Entry nameiPNP_SHEPC
    AccessioniPrimary (citable) accession number: A4Y9B5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3