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A4Y9B5

- PNP_SHEPC

UniProt

A4Y9B5 - PNP_SHEPC

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851MagnesiumUniRule annotation
Metal bindingi491 – 4911MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-2924-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Sputcn32_2829
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000006699: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 699699Polyribonucleotide nucleotidyltransferasePRO_0000329843Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi319224.Sputcn32_2829.

Structurei

3D structure databases

ProteinModelPortaliA4Y9B5.
SMRiA4Y9B5. Positions 616-691.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61160KHUniRule annotationAdd
BLAST
Domaini621 – 68969S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4Y9B5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPIVKSFEY GQHTVTLETG VIARQADAAV LASMGDTTVL VTVVGKKEAE
60 70 80 90 100
AGRDFFPLTV NYQEKTYAAG KIPGGFFKRE GRPSEDETLI ARLIDRPIRP
110 120 130 140 150
LFPNGFKNEV QVIITVVSVD PQIEPDIISM IGTSAALAIS GIPFSGPLGA
160 170 180 190 200
ARVGYINGEY VLNPTVAQIE TSQLNLVVAG TASAVLMVES EAQALPEEVM
210 220 230 240 250
LGSVVYGHDQ QQVVINAIAE FKAEAGKPMW DWTAPVQDET LVAQIKELAE
260 270 280 290 300
AGLGDAYKIQ VKQDRYAQVA VVKAATKEAL LASNPNIDLR EVDNLLGSLE
310 320 330 340 350
KKVVRGRIIR GEPRIDGREP DMIRALSVLA GVLPRTHGSA LFTRGETQAL
360 370 380 390 400
VTCTLGTERD AQKIDSIMGE RTNRFMLHYN FPPYSVGETG MVGSPKRREI
410 420 430 440 450
GHGKLAWRGI NAVMPSAEEF PYSVRVVSEI TESNGSSSMA SVCGTSLALM
460 470 480 490 500
DAGVPIKTSV AGIAMGLVKE GDDFVVLSDI LGDEDHLGDM DFKVAGTRGG
510 520 530 540 550
ITALQMDIKI EGITKEIMEI ALQQAYGARV HILNVMDQAI GSHRDDISDH
560 570 580 590 600
APRITTIKIN PEKIRDVIGK GGAVIRALTE ETGTTIELED DGTVRIASSN
610 620 630 640 650
GEATKEAIRR IEEITSEVEV GRIYNGKVIR IVDFGAFVNI LPGKDGLVHI
660 670 680 690
SQISDERVAN VSDHLELNQE VAVKVMEVDR QGRVRLSIKE AQTKEAAAE
Length:699
Mass (Da):75,453
Last modified:May 29, 2007 - v1
Checksum:i84ACB5302ECC4249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP76548.1.
RefSeqiYP_001184347.1. NC_009438.1.

Genome annotation databases

EnsemblBacteriaiABP76548; ABP76548; Sputcn32_2829.
GeneIDi5078661.
KEGGispc:Sputcn32_2829.
PATRICi23554626. VBIShePut135485_2957.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP76548.1 .
RefSeqi YP_001184347.1. NC_009438.1.

3D structure databases

ProteinModelPortali A4Y9B5.
SMRi A4Y9B5. Positions 616-691.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 319224.Sputcn32_2829.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP76548 ; ABP76548 ; Sputcn32_2829 .
GeneIDi 5078661.
KEGGi spc:Sputcn32_2829.
PATRICi 23554626. VBIShePut135485_2957.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci SPUT319224:GHAP-2924-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CN-32 / ATCC BAA-453.

Entry informationi

Entry nameiPNP_SHEPC
AccessioniPrimary (citable) accession number: A4Y9B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 29, 2007
Last modified: November 26, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3