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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei93S-adenosyl-L-methionineUniRule annotation1
Binding sitei120S-adenosyl-L-methionineUniRule annotation1
Active sitei143By similarity1
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Binding sitei147SubstrateUniRule annotation1
Binding sitei179SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSPUT319224:G1G8R-2814-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Sputcn32_2699
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000006699 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000644101 – 238tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST238

Interactioni

Protein-protein interaction databases

STRINGi319224.Sputcn32_2699

Structurei

3D structure databases

ProteinModelPortaliA4Y8Y5
SMRiA4Y8Y5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 219Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A4Y8Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVTTAEFN EEGKYLRKIR SFVLREGRLT KGQAQAIESQ WPTMGLDYSP
60 70 80 90 100
VPLNLSEVFG READTVLEIG FGMGASLVQM AKDAPEQNFI GIEVHKPGVG
110 120 130 140 150
SCLSDAAIAG VTNLRVYHHD AMEVLEHAIA DGSLARVQLF FPDPWHKKRH
160 170 180 190 200
HKRRIVQAEF AELVRRKLKI GGVFHMATDW EEYSEHMLEV MNAAPGYKNQ
210 220 230
SADGTVVPRP DHRPLTKFEA RGHRLGHGVW DLMFERIA
Length:238
Mass (Da):26,814
Last modified:May 29, 2007 - v1
Checksum:i57E68FD2E3DFEFEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA Translation: ABP76418.1
RefSeqiWP_011919684.1, NC_009438.1

Genome annotation databases

EnsemblBacteriaiABP76418; ABP76418; Sputcn32_2699
KEGGispc:Sputcn32_2699

Similar proteinsi

Entry informationi

Entry nameiTRMB_SHEPC
AccessioniPrimary (citable) accession number: A4Y8Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: April 25, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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