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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi98 – 981ZincUniRule annotation
Metal bindingi100 – 1001ZincUniRule annotation
Metal bindingi125 – 1251ZincUniRule annotation
Metal bindingi127 – 1271ZincUniRule annotation
Binding sitei239 – 2391ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-2575-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Sputcn32_2499
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000006699 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Glutamate--tRNA ligasePRO_1000001960Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi319224.Sputcn32_2499.

Structurei

3D structure databases

ProteinModelPortaliA4Y8D7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi9 – 1911"HIGH" regionAdd
BLAST
Motifi236 – 2405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiIEAFKWV.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4Y8D7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKTRFAPS PTGFLHVGGA RTALYSWLQA RANNGEFVLR IEDTDIERST
60 70 80 90 100
QAACDAILEG MNWLGLTWDE GPYYQTKRFD RYNEIIAQML EKGTAYKCYC
110 120 130 140 150
SRERIDALRE SQAANGEAQK YDGCCRDLPA RDTDEPFVVR FKNPIGGSVV
160 170 180 190 200
FDDHVRGRIE FSNDALDDLI IARTDGVPTY NFCVVVDDWD MGITCVVRGE
210 220 230 240 250
DHINNTPRQI NILKALGAPI PEYAHVSMIL GDDGAKLSKR HGAVSVMQYR
260 270 280 290 300
DDGYLPEALL NYLVRLGWSH GDQEVFSLEE MKQYFKLDDI NKAPSAFNTD
310 320 330 340 350
KLVWLNQHYI KTLDPEYVAT HLQWHMDDQK IDTSNGPALA EVVTALAERA
360 370 380 390 400
KTLKELAASS RYFYEDFADF DAEQAKKHLR GVALEPLQLV QQKLAALTEW
410 420 430 440 450
TVEAIHLAIE QTATELDVGM GKVGMPLRVA VTGAGQSPGL DITLFLIGRS
460
RSEQRISKAI EFVADRINS
Length:469
Mass (Da):52,640
Last modified:May 29, 2007 - v1
Checksum:i8B781E80C88C527E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP76220.1.
RefSeqiWP_011919558.1. NC_009438.1.
YP_001184019.1. NC_009438.1.

Genome annotation databases

EnsemblBacteriaiABP76220; ABP76220; Sputcn32_2499.
KEGGispc:Sputcn32_2499.
PATRICi23553886. VBIShePut135485_2605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP76220.1.
RefSeqiWP_011919558.1. NC_009438.1.
YP_001184019.1. NC_009438.1.

3D structure databases

ProteinModelPortaliA4Y8D7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi319224.Sputcn32_2499.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP76220; ABP76220; Sputcn32_2499.
KEGGispc:Sputcn32_2499.
PATRICi23553886. VBIShePut135485_2605.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiIEAFKWV.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-2575-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CN-32 / ATCC BAA-453.

Entry informationi

Entry nameiSYE_SHEPC
AccessioniPrimary (citable) accession number: A4Y8D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: May 27, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.