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A4Y897

- FADJ_SHEPC

UniProt

A4Y897 - FADJ_SHEPC

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei116 – 1161Important for catalytic activityUniRule annotation
    Sitei138 – 1381Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSPUT319224:GHAP-2526-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:Sputcn32_2459
    OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
    Taxonomic identifieri319224 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000006699: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 706706Fatty acid oxidation complex subunit alphaPRO_0000323529Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi319224.Sputcn32_2459.

    Structurei

    3D structure databases

    ProteinModelPortaliA4Y897.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 188188Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni308 – 7063993-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OMAiPFRYMDT.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4Y897-1 [UniParc]FASTAAdd to Basket

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    MEKTFNLTRR DDGIAILTMD VPGETMNTLK AQFGPEISEI LAEIKSDPHI    50
    RGLVLISGKK DSFVAGADIS MLDACKTAGD AKALSQQGHV VFNELEALKI 100
    PVVAAIHGAC LGGGLELALA CHQRVCSDDG KTMLGVPEVQ LGLLPGGGGT 150
    QRLPRLVGIT TALDMMLTGK QIRPKQALKM GLVNDVVPQT ILLQTAVEMA 200
    LAGKRAPKPI KKSLVNQVLE GTSFGRNIIF DQATKQVEKK TQGNYPAPAK 250
    IIDCVRQGIA KGMQKGLEVE ASHFAELVVS KESEALRSIF FATTEMKKET 300
    GAEGATPRKV KKAVILGGGL MGGGIASVTT TKAKIPVRVK DISEKGLSNA 350
    LAYAYKLLDK GVKRRHMTPA VRDNLMALMT TTTEYKGVKD ADIVVEAVFE 400
    DLALKHQMVK DIERECGEHT IFASNTSSLP ISQIAEAATR PENVIGLHYF 450
    SPVEKMPLVE VIAHAKTSPE TIATTVAFAR KQGKTPIVVQ DGAGFYVNRI 500
    LALYMNEAAQ LLLEGQSVEH LDKALVKFGF PVGPITLLDE VGIDVGAKIS 550
    PILDKELGER FKAPAAFDKL LGDDRKGRKN GKGFYQYGAS SKKTKAVDET 600
    VYGVLGIKPS TNKDAKALAE RCVVQMLNEA VRCLDEGIIA SPRDGDIGAI 650
    FGIGFPPFLG GPFHYIDTLG AANLVKILEG YQSQFGNRFE PCERLKTMAR 700
    ENVSFF 706
    Length:706
    Mass (Da):75,966
    Last modified:May 29, 2007 - v1
    Checksum:i700BB3896BE566F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP76180.1.
    RefSeqiWP_011919536.1. NC_009438.1.
    YP_001183979.1. NC_009438.1.

    Genome annotation databases

    EnsemblBacteriaiABP76180; ABP76180; Sputcn32_2459.
    GeneIDi5078593.
    KEGGispc:Sputcn32_2459.
    PATRICi23553788. VBIShePut135485_2565.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP76180.1 .
    RefSeqi WP_011919536.1. NC_009438.1.
    YP_001183979.1. NC_009438.1.

    3D structure databases

    ProteinModelPortali A4Y897.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 319224.Sputcn32_2459.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP76180 ; ABP76180 ; Sputcn32_2459 .
    GeneIDi 5078593.
    KEGGi spc:Sputcn32_2459.
    PATRICi 23553788. VBIShePut135485_2565.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OMAi PFRYMDT.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci SPUT319224:GHAP-2526-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CN-32 / ATCC BAA-453.

    Entry informationi

    Entry nameiFADJ_SHEPC
    AccessioniPrimary (citable) accession number: A4Y897
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3