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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Important for catalytic activityUniRule annotation1
Sitei138Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciSPUT319224:G1G8R-2553-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:Sputcn32_2459
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000006699 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003235291 – 706Fatty acid oxidation complex subunit alphaAdd BLAST706

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi319224.Sputcn32_2459

Structurei

3D structure databases

ProteinModelPortaliA4Y897
SMRiA4Y897
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 188Enoyl-CoA hydrataseUniRule annotationAdd BLAST188
Regioni308 – 7063-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST399

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261346
KOiK01782
OMAiPFRYMDT
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit

Sequencei

Sequence statusi: Complete.

A4Y897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKTFNLTRR DDGIAILTMD VPGETMNTLK AQFGPEISEI LAEIKSDPHI
60 70 80 90 100
RGLVLISGKK DSFVAGADIS MLDACKTAGD AKALSQQGHV VFNELEALKI
110 120 130 140 150
PVVAAIHGAC LGGGLELALA CHQRVCSDDG KTMLGVPEVQ LGLLPGGGGT
160 170 180 190 200
QRLPRLVGIT TALDMMLTGK QIRPKQALKM GLVNDVVPQT ILLQTAVEMA
210 220 230 240 250
LAGKRAPKPI KKSLVNQVLE GTSFGRNIIF DQATKQVEKK TQGNYPAPAK
260 270 280 290 300
IIDCVRQGIA KGMQKGLEVE ASHFAELVVS KESEALRSIF FATTEMKKET
310 320 330 340 350
GAEGATPRKV KKAVILGGGL MGGGIASVTT TKAKIPVRVK DISEKGLSNA
360 370 380 390 400
LAYAYKLLDK GVKRRHMTPA VRDNLMALMT TTTEYKGVKD ADIVVEAVFE
410 420 430 440 450
DLALKHQMVK DIERECGEHT IFASNTSSLP ISQIAEAATR PENVIGLHYF
460 470 480 490 500
SPVEKMPLVE VIAHAKTSPE TIATTVAFAR KQGKTPIVVQ DGAGFYVNRI
510 520 530 540 550
LALYMNEAAQ LLLEGQSVEH LDKALVKFGF PVGPITLLDE VGIDVGAKIS
560 570 580 590 600
PILDKELGER FKAPAAFDKL LGDDRKGRKN GKGFYQYGAS SKKTKAVDET
610 620 630 640 650
VYGVLGIKPS TNKDAKALAE RCVVQMLNEA VRCLDEGIIA SPRDGDIGAI
660 670 680 690 700
FGIGFPPFLG GPFHYIDTLG AANLVKILEG YQSQFGNRFE PCERLKTMAR

ENVSFF
Length:706
Mass (Da):75,966
Last modified:May 29, 2007 - v1
Checksum:i700BB3896BE566F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA Translation: ABP76180.1
RefSeqiWP_011919536.1, NC_009438.1

Genome annotation databases

EnsemblBacteriaiABP76180; ABP76180; Sputcn32_2459
KEGGispc:Sputcn32_2459

Similar proteinsi

Entry informationi

Entry nameiFADJ_SHEPC
AccessioniPrimary (citable) accession number: A4Y897
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 29, 2007
Last modified: April 25, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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