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A4Y7Y3

- BIOB_SHEPC

UniProt

A4Y7Y3 - BIOB_SHEPC

Protein

Biotin synthase

Gene

bioB

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi191 – 1911Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi263 – 2631Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSPUT319224:GHAP-2407-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Sputcn32_2345
    OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
    Taxonomic identifieri319224 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000006699: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350Biotin synthasePRO_0000381623Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi319224.Sputcn32_2345.

    Structurei

    3D structure databases

    ProteinModelPortaliA4Y7Y3.
    SMRiA4Y7Y3. Positions 7-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4Y7Y3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQLQVRHDW KREEIEALFA LPMNDLLFKA HSIHREVYDP NEVQISRLLS    50
    IKTGACPEDC KYCPQSARYD TGLEKERLLA METVLTEARS AKAAGASRFC 100
    MGAAWRNPKE KDMPYLKQMV QEVKALGMET CMTLGMLSAE QANELADAGL 150
    DYYNHNLDTS PEYYGDVITT RTYQNRLDTL THVRASGMKV CSGGIVGMGE 200
    KATDRAGLLQ QLANLPQHPD SVPINMLVKV AGTPFEKLDD LDPLEFVRTI 250
    AVARILMPLS RVRLSAGREN MSDELQAMCF FAGANSIFYG CKLLTTPNPE 300
    ESDDMGLFRR LGLRPEQGAV ATLDDEQAVL AKAAAHQDKS TAQFYDAAAL 350
    Length:350
    Mass (Da):38,820
    Last modified:May 29, 2007 - v1
    Checksum:i377335D2F42C6D32
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP76066.1.
    RefSeqiYP_001183865.1. NC_009438.1.

    Genome annotation databases

    EnsemblBacteriaiABP76066; ABP76066; Sputcn32_2345.
    GeneIDi5080368.
    KEGGispc:Sputcn32_2345.
    PATRICi23553542. VBIShePut135485_2447.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP76066.1 .
    RefSeqi YP_001183865.1. NC_009438.1.

    3D structure databases

    ProteinModelPortali A4Y7Y3.
    SMRi A4Y7Y3. Positions 7-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 319224.Sputcn32_2345.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP76066 ; ABP76066 ; Sputcn32_2345 .
    GeneIDi 5080368.
    KEGGi spc:Sputcn32_2345.
    PATRICi 23553542. VBIShePut135485_2447.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci SPUT319224:GHAP-2407-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CN-32 / ATCC BAA-453.

    Entry informationi

    Entry nameiBIOB_SHEPC
    AccessioniPrimary (citable) accession number: A4Y7Y3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3