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A4Y4U3

- GLGB_SHEPC

UniProt

A4Y4U3 - GLGB_SHEPC

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei416 – 4161NucleophileUniRule annotation
    Active sitei469 – 4691Proton donorUniRule annotation

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciSPUT319224:GHAP-1272-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
    Alpha-(1->4)-glucan branching enzymeUniRule annotation
    Glycogen branching enzymeUniRule annotation
    Short name:
    BEUniRule annotation
    Gene namesi
    Name:glgBUniRule annotation
    Ordered Locus Names:Sputcn32_1248
    OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
    Taxonomic identifieri319224 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000006699: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7457451,4-alpha-glucan branching enzyme GlgBPRO_1000044999Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi319224.Sputcn32_1248.

    Structurei

    3D structure databases

    ProteinModelPortaliA4Y4U3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiAKLLFMG.
    OrthoDBiEOG6JX7GT.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4Y4U3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMTQANTYFY NGADVALLNG QYTDVFSLLG MHTSEDGKSL IVRCFLRNAF    50
    KVDVISIKDG RRVAGLEKVN DAGLFAGTMG RRVKPFLYLL RIQYPLSQIE 100
    IVDPYQFGPL LNADDLYLFG EGSAERAYKF LGANWRDVEG IEGVHFCVWA 150
    PNAKRVSVVG DFNHWDDTQH VMRQHMANGL WEIFLPNVAE GAHYKFDLVH 200
    PNGERHAKSD PMATQMECAP HNASIVPKKS KHSWKDTLWM NKRAVTAWHK 250
    APMAIYEVHL GSWRRKGDNG EQYLDYQDLI EQLIPYVKAQ GFTHIELMPI 300
    SEYPFDGSWG YQPVGLYAPT HRFGDANGLK AFVDACHQAD IGVVLDWVAA 350
    HFPKDPHGLV RFDGTCLYEH EDPRKGTHPD WDTLIYNYGR GEVRSFLLSN 400
    ACYWLREFHF DGLRIDAVSS MLYLDYSREP GQWLPNAYGG RENLEAISFL 450
    QMLNQRLYQA FPGICMIAEE STAFAGVTKP TDHHGLGFGF KWNMGWMNDS 500
    LSYLSRDPIY RQYHHHQLTF SLMYAYTEQF MLSVSHDEVV HGKGSLLHKI 550
    PGDDWQKFAT LRAYYGFMWG HPGKKLLFMG SEFAQRDEWD HNHSLDWHLL 600
    AFEPHQGVQR WLKDLNQLYQ GMSALSVLDY QPAGFRWLDC DNGSASIFTF 650
    VRYGLAGDAP LVFVINMTPS VHHGFRIGLP QAGDFCEYLN SDSYLYCGSN 700
    QGNAGLVVAQ NQPWQGMESS ALITVPPLSC LVLGLAANQV EAKRR 745
    Length:745
    Mass (Da):84,799
    Last modified:May 29, 2007 - v1
    Checksum:i0D9CB27E3F405667
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP74976.1.
    RefSeqiYP_001182775.1. NC_009438.1.

    Genome annotation databases

    EnsemblBacteriaiABP74976; ABP74976; Sputcn32_1248.
    GeneIDi5081560.
    KEGGispc:Sputcn32_1248.
    PATRICi23551139. VBIShePut135485_1283.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000681 Genomic DNA. Translation: ABP74976.1 .
    RefSeqi YP_001182775.1. NC_009438.1.

    3D structure databases

    ProteinModelPortali A4Y4U3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 319224.Sputcn32_1248.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP74976 ; ABP74976 ; Sputcn32_1248 .
    GeneIDi 5081560.
    KEGGi spc:Sputcn32_1248.
    PATRICi 23551139. VBIShePut135485_1283.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi AKLLFMG.
    OrthoDBi EOG6JX7GT.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci SPUT319224:GHAP-1272-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CN-32 / ATCC BAA-453.

    Entry informationi

    Entry nameiGLGB_SHEPC
    AccessioniPrimary (citable) accession number: A4Y4U3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3