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A4Y4A4

- SYI_SHEPC

UniProt

A4Y4A4 - SYI_SHEPC

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Sputcn32_1059
Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-1081-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Sputcn32_1059
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000006699: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022119Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi319224.Sputcn32_1059.

Structurei

3D structure databases

ProteinModelPortaliA4Y4A4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4Y4A4-1 [UniParc]FASTAAdd to Basket

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MSDYKFTLNL PETEFPMRGN LANREPEMLE RWTKDGLYQQ IRDSRIGRTP    50
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKTMSGFDAP YVPGWDCHGL 100
PIELKVEQKV GKPGQKITAA EFREECRKYA AEQVNGQRED FIRLGVLGDW 150
QNPYLTMDFS TEANIVRSLS KVIESGHLHK GVKPVHWCTD CGSALAEAEV 200
EYEDKTSPAI DVAFVAADSK AVAAKFGVSD YSHPVSMVIW TTTPWTLPAN 250
RALSLSPELD YSLVEFEKDG VTQALILAEV LVESCLTRYN VESHTVLGTT 300
KGAALELVCF NHPFLDFDVP AILGDHVTTD AGTGIVHTAP GHGQDDFVVG 350
QKYGLEVANP VGDNGVYKPD TKYFAGQHVF KANDNVVALL REKGALLNHV 400
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN HGLRTQALKE IEQTQWIPDW 450
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VNRETEELHP DSVSLMERVA 500
NRIEQQGIQA WWDLDAAELL GDEADQYRKV TDTLDVWYDS GSTFSSVVAA 550
RPEFHGHGVD LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
GKGRKMSKSI GNVIAPQTVT NKLGADILRL WVAATDYSGE MTVSDEILNR 650
SADAYRRIRN TARFLLANLN GFDPAKDLVA VEDMVALDRW VVRRAAALQQ 700
EIIEAYDQYN FHIVTQKLMQ FCSVELGSFY LDIIKDRQYT AKQEGHARRS 750
CQSALFHIAE AMVRWIAPVL SFTADEVWQL LPGERDAYVF TQEWYQGLKS 800
VTLATDLSDD YWQQLLAVRN EVNKVIEQAR RDKRIGGSLE AEVTLFADAT 850
LTEQLTHIGD ELRFVLLTSE AKVLPLADAT TEAVETELAS LKLVVASSTA 900
EKCERCWHHR EEVGTIEAHP TLCTRCVTNI EGDGEVRQFA 940
Length:940
Mass (Da):105,682
Last modified:May 29, 2007 - v1
Checksum:i73701EA678966B3D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000681 Genomic DNA. Translation: ABP74787.1.
RefSeqiYP_001182586.1. NC_009438.1.

Genome annotation databases

EnsemblBacteriaiABP74787; ABP74787; Sputcn32_1059.
GeneIDi5081667.
KEGGispc:Sputcn32_1059.
PATRICi23550737. VBIShePut135485_1083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000681 Genomic DNA. Translation: ABP74787.1 .
RefSeqi YP_001182586.1. NC_009438.1.

3D structure databases

ProteinModelPortali A4Y4A4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 319224.Sputcn32_1059.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP74787 ; ABP74787 ; Sputcn32_1059 .
GeneIDi 5081667.
KEGGi spc:Sputcn32_1059.
PATRICi 23550737. VBIShePut135485_1083.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPUT319224:GHAP-1081-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CN-32 / ATCC BAA-453.

Entry informationi

Entry nameiSYI_SHEPC
AccessioniPrimary (citable) accession number: A4Y4A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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