A4Y3Y0 (RLMG_SHEPC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Sputcn32_0934

Organism

Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) [Complete proteome] [HAMAP]

Taxonomic identifier

319224 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence ABP74663.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366514

Sequences

	Sequence		Length	Mass (Da)
	A4Y3Y0 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 016A84D5FE06484F	FASTA	378	41,857
	10 20 30 40 50 60 MTTQFSVAGV ELELLRYPAQ QESNLQAWDA ADEHLLKSLI ESEQAAVPTA IVNDSFGALS 70 80 90 100 110 120 CGLSKLHPSW PLFVETDART SFLGTEQNHG RNQLPMDNLQ RFTSRDTLPE NLALVLMKLP 130 140 150 160 170 180 KNLSYFAHQL TRLSQVLPAG TRVLVAAKAK SINSALLDIF AKHLGPASAS LAWKNTRVIT 190 200 210 220 230 240 CVSDGKPRPL AKEVTWAVPE YQLEISNLSN VFAANKLDIG ARIMLENLPK GNFKSIVDLG 250 260 270 280 290 300 CGNGVLGLRT AQLSPEADIH FIDDSEMAVA SAKANWARNQ LPADKGHFYW DDCMTHLPEE 310 320 330 340 350 360 VQPDLVLCNP PFHQGEAITD HIAWQMFLDA RRRLKEGGIL HIVGNRHLAY HVKLQRLFKN 370 CTTVASNGKF VILQAQKK

References

[1]

"Complete sequence of Shewanella putrefaciens CN-32." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Fredrickson J., Tiedje J., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CN-32 / ATCC BAA-453.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000681 Genomic DNA. Translation: ABP74663.1. Different initiation.
RefSeq	YP_001182462.1. NC_009438.1.
3D structure databases
ProteinModelPortal	A4Y3Y0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4Y3Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5081846.
GenomeReviews	Gene locus Sputcn32_0934 in contig CP000681_GR.
KEGG	spc:Sputcn32_0934.
NMPDR	fig|319224.13.peg.840.
PATRIC	23550471. VBIShePut135485_0956.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
ProtClustDB	CLSK906089.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHEPC

Accession

Primary (citable) accession number: A4Y3Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program