A4Y2G4 (KATG_SHEPC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Sputcn32_0415

Organism

Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) [Complete proteome] [HAMAP]

Taxonomic identifier

319224 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Catalase-peroxidase HAMAP MF_01961		PRO_0000354922
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4Y2G4 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: D34F34094D910084	FASTA	728	81,303
	10 20 30 40 50 60 MDKTHSSQGK CPVMHGGNTS VTTNNMDWWP KALNLDILHQ HDKKTDPMDP TFNYSEAFKT 70 80 90 100 110 120 LDLAAVKQDL YALMTDSQDW WPADWGHYGG LMIRMAWHSA GTYRIADGRG GAGTGNQRFA 130 140 150 160 170 180 PLNSWPDNAN LDKARRLLWP IKKKYGNKLS WADLIILAGN VAYESMGLKT YGFAGGRADI 190 200 210 220 230 240 WHPEKDIYWG SEKQWLAPSD NPNSRYSGER DLENPLAAVM MGLIYVNPEG VDGNPDPLRT 250 260 270 280 290 300 AQDIRITFAR MAMDDEETVA LTAGGHTVGK CHGNGKAQNL GPEPEAEDVE AQGLGWLNKH 310 320 330 340 350 360 GRGVGRNTVT SGIEGAWTTH PTQWDNGYFS LLLGYDWELK KSPAGAWQWE PINIKEEDKP 370 380 390 400 410 420 VDVEDPTIRH NPIMTDADMA MKMDPEYRKI SEKFYKDPAY FSEVFARAWF KLTHRDLGPK 430 440 450 460 470 480 SRYLGPEVPA EDLIWQDPIP QVDYRLSDDD ITALKAKILA SGLSIAELVT TAWDSARTFR 490 500 510 520 530 540 GSDYRGGANG ARIRLAPQKD WEGNEPVRLQ KVLKVLTDIQ TGLSQKVSIA DLIVLGGTAA 550 560 570 580 590 600 VEKAAQDAGV NIRVPFASGR GDASQEMTDI ESFAVLEPLH DAYRNWQKKD YVVQPEELML 610 620 630 640 650 660 DRTQLMGLTA HEMTVLIGGM RVLGANYAGS AHGVFTDRVG VLSNDFFVNL TDMSYNWKPA 670 680 690 700 710 720 GNNLYQIIER KTGTVKWTAT RVDLVFGSNS VLRSYAEIYA QDDAKEKFVH DFVNAWTKVM NADRFDLA

References

[1]

"Complete sequence of Shewanella putrefaciens CN-32." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Fredrickson J., Tiedje J., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CN-32 / ATCC BAA-453.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000681 Genomic DNA. Translation: ABP74147.1.
RefSeq	YP_001181946.1. NC_009438.1.
3D structure databases
ProteinModelPortal	A4Y2G4.
SMR	A4Y2G4. Positions 27-727.
ModBase	Search...
Protein-protein interaction databases
STRING	A4Y2G4.
Protein family/group databases
PeroxiBase	3615. SputCP01_CN-32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5081399.
GenomeReviews	Gene locus Sputcn32_0415 in contig CP000681_GR.
KEGG	spc:Sputcn32_0415.
PATRIC	23549382. VBIShePut135485_0420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	A4Y2G4.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SHEPC

Accession

Primary (citable) accession number: A4Y2G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program