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Protein

Ribosomal RNA large subunit methyltransferase F

Gene

rlmF

Organism
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the adenine in position 1618 of 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-132-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase FUniRule annotation (EC:2.1.1.181UniRule annotation)
Alternative name(s):
23S rRNA mA1618 methyltransferaseUniRule annotation
rRNA adenine N-6-methyltransferaseUniRule annotation
Gene namesi
Name:rlmFUniRule annotation
Ordered Locus Names:Sputcn32_0128
OrganismiShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
Taxonomic identifieri319224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000006699 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Ribosomal RNA large subunit methyltransferase FPRO_0000349960Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi319224.Sputcn32_0128.

Structurei

3D structure databases

ProteinModelPortaliA4Y1N1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. METTL16/RlmF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EBA. Bacteria.
COG3129. LUCA.
HOGENOMiHOG000218605.
KOiK06970.
OMAiVTLCNPP.
OrthoDBiPOG091H05H4.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F. 1 hit.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

A4Y1N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKPPRSTQI LSCNAPNGKP KTQHPSARAK VKRTPVNTRS IAEIKKALHP
60 70 80 90 100
RNVHINGYDF NALIKAFPRL NAFVRPTSFG GLSIDFADPE AVKTLNTALL
110 120 130 140 150
KHHYGIDFWD IPKGALCPPI PGRVDYLHYL ADLLAEGDHH LVMDRVSVLD
160 170 180 190 200
IGTGANGIYP ILGCQVFGWH FVASDINSIS LANVQGIIAQ NPALHGRLNL
210 220 230 240 250
RLQGDESAIF KGVIQPQERF ELTLCNPPFH ASLAEAAEGS LRKVRNLQLN
260 270 280 290 300
RGRTAKPVAK LNFGGQGAEL WCQGGEPQFL ATMIDESQAF ADQCLWFTSL
310 320 330 340 350
VSKKENLKPC YQALAKLAVD TVKTIEMQQG NKMTRILAWS FQSAAKRKIW

RNAHLSD
Length:357
Mass (Da):39,438
Last modified:May 29, 2007 - v1
Checksum:iBFD1A92ED24C8ED4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP73864.1.
RefSeqiWP_011791170.1. NC_009438.1.

Genome annotation databases

EnsemblBacteriaiABP73864; ABP73864; Sputcn32_0128.
KEGGispc:Sputcn32_0128.
PATRICi23548804. VBIShePut135485_0135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000681 Genomic DNA. Translation: ABP73864.1.
RefSeqiWP_011791170.1. NC_009438.1.

3D structure databases

ProteinModelPortaliA4Y1N1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi319224.Sputcn32_0128.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP73864; ABP73864; Sputcn32_0128.
KEGGispc:Sputcn32_0128.
PATRICi23548804. VBIShePut135485_0135.

Phylogenomic databases

eggNOGiENOG4105EBA. Bacteria.
COG3129. LUCA.
HOGENOMiHOG000218605.
KOiK06970.
OMAiVTLCNPP.
OrthoDBiPOG091H05H4.

Enzyme and pathway databases

BioCyciSPUT319224:GHAP-132-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F. 1 hit.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLMF_SHEPC
AccessioniPrimary (citable) accession number: A4Y1N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.