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Protein

Thiazole synthase

Gene

thiG

Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106Schiff-base intermediate with DXPUniRule annotation1
Binding sitei167DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciPMEN399739:G1G8Q-4253-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:Pmen_4167
OrganismiPseudomonas mendocina (strain ymp)
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000229 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000260291 – 264Thiazole synthaseAdd BLAST264

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi399739.Pmen_4167

Structurei

3D structure databases

ProteinModelPortaliA4XZZ8
SMRiA4XZZ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni193 – 194DXP bindingUniRule annotation2
Regioni215 – 216DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.701 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

A4XZZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVRSDKPF TLAGRTYQSR LLVGTGKYKD MDETRDAIAA SGAEIVTVAV
60 70 80 90 100
RRTNIGQNPG EPNLLDVISP EQYTILPNTA GCYDAAEAVR TCRLARELLD
110 120 130 140 150
GHKLVKLEVL ADQKTLFPNV IETIKAAEIL VKDGFDVMVY TSDDPIIARQ
160 170 180 190 200
LAEIGCIAVM PLAGLIGSGL GICNPYNLRI ILEEAKVPVL VDAGVGTASD
210 220 230 240 250
ATIAMELGCE AVLLNSAIAH AQDPILMARA MKHAVEAGRL AYLAGRMPKK
260
LYASASSPLD GLIK
Length:264
Mass (Da):28,175
Last modified:May 29, 2007 - v1
Checksum:iFE92D03C2BE251FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA Translation: ABP86914.1
RefSeqiWP_012019979.1, NC_009439.1

Genome annotation databases

EnsemblBacteriaiABP86914; ABP86914; Pmen_4167
GeneIDi5105686
KEGGipmy:Pmen_4167
PATRICifig|399739.8.peg.4218

Similar proteinsi

Entry informationi

Entry nameiTHIG_PSEMY
AccessioniPrimary (citable) accession number: A4XZZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: February 28, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome