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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei632 – 6321ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMEN399739:GHR6-3849-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Pmen_3786
OrganismiPseudomonas mendocina (strain ymp)
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000229: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870Leucine--tRNA ligasePRO_0000334794Add
BLAST

Proteomic databases

PRIDEiA4XYW8.

Interactioni

Protein-protein interaction databases

STRINGi399739.Pmen_3786.

Structurei

3D structure databases

ProteinModelPortaliA4XYW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi629 – 6335"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4XYW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEQYSPREI EAAAQSHWDA QKSFVVSEQP GKDTFYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VIARYQRMQG KNVLQPMGWD AFGMPAENAA MKNQVAPAKW
110 120 130 140 150
TYENIAYMKS QLKSLGLAID WTREVTTCKP DYYRWEQWLF TRLFEKGVIY
160 170 180 190 200
RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAD
210 220 230 240 250
ELLSSLDELD GWPEQVKTMQ RNWIGKSFGA DIVFDYDVAS IGIDGQLKVY
260 270 280 290 300
STRPDTLMGA TYVAVAAEHP LAQRAAESNP AIAAFIAECK AGSVAEADMA
310 320 330 340 350
TMEKKGLATG QFVIHPLTGD KLPVFVANYV LWGYGEGAVM AVPAHDERDF
360 370 380 390 400
EFANKYDLPI KQVYTGEGKD YDASTWQDWY GDKAGLTTIN SGKYDGLDFS
410 420 430 440 450
AAFDAIVGDL EASAHGARKT QFRLRDWGIS RQRYWGCPIP IIHCDSCGDV
460 470 480 490 500
PVPEDQLPVV LPEDVVPDGA GSPLAKMPEF YECTCPKCGA PAKRETDTMD
510 520 530 540 550
TFVESSWYFA RYASPNYEGG MVDPQAANHW LPVDQYIGGI EHAILHLLYA
560 570 580 590 600
RFFHKLMRDE GLVSSNEPFK NLLTQGMVVA ETYYRTLENG GKDWFNPADV
610 620 630 640 650
EVERDAKAKV IGAKLKSDGL PVEIGGTEKM SKSKNNGVDP QDMIDAYGAD
660 670 680 690 700
TCRLFMMFAS PPDMSCEWSD AGVEGANRFL RRVWRLAHGH VSAGLPGKLD
710 720 730 740 750
VASLSDEQKA VRRAIHLAIK QASNDIGQHH KFNTAIAQVM TLMNVLEKAA
760 770 780 790 800
TATEQDRALV QEGLEIVTLL LAPITPHISH ELWQRLGHAD AVIDAQWPQV
810 820 830 840 850
DESALVQDSL TLVVQVNGKL RGQIEVPASA SREDVEAAAR ANENVLRFTE
860 870
GLSIRKVIVV PGKLVNIVAN
Length:870
Mass (Da):96,711
Last modified:May 20, 2008 - v2
Checksum:i068FCFFC99162D69
GO

Sequence cautioni

The sequence ABP86534.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA. Translation: ABP86534.1. Different initiation.
RefSeqiYP_001189266.1. NC_009439.1.

Genome annotation databases

EnsemblBacteriaiABP86534; ABP86534; Pmen_3786.
GeneIDi5106017.
KEGGipmy:Pmen_3786.
PATRICi19914208. VBIPseMen131592_3839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA. Translation: ABP86534.1. Different initiation.
RefSeqiYP_001189266.1. NC_009439.1.

3D structure databases

ProteinModelPortaliA4XYW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399739.Pmen_3786.

Proteomic databases

PRIDEiA4XYW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP86534; ABP86534; Pmen_3786.
GeneIDi5106017.
KEGGipmy:Pmen_3786.
PATRICi19914208. VBIPseMen131592_3839.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPMEN399739:GHR6-3849-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ymp.

Entry informationi

Entry nameiSYL_PSEMY
AccessioniPrimary (citable) accession number: A4XYW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.