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Protein

Acetate kinase

Gene

ackA

Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (Pmen_3645)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Binding sitei17ATPUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Active sitei144Proton donor/acceptorUniRule annotation1
Sitei176Transition state stabilizerUniRule annotation1
Sitei237Transition state stabilizerUniRule annotation1
Metal bindingi381MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 208ATPUniRule annotation5
Nucleotide bindingi279 – 281ATPUniRule annotation3
Nucleotide bindingi327 – 331ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMEN399739:G1G8Q-3716-MONOMER.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:Pmen_3644
OrganismiPseudomonas mendocina (strain ymp)
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000229 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000022481 – 394Acetate kinaseAdd BLAST394

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi399739.Pmen_3644.

Structurei

3D structure databases

ProteinModelPortaliA4XYH6.
SMRiA4XYH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiFMMQKEN.
OrthoDBiPOG091H02K4.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiView protein in InterPro
IPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiView protein in Pfam
PF00871. Acetate_kinase. 1 hit.
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiView protein in PROSITE
PS01075. ACETATE_KINASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

A4XYH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARNILVIN CGSSSIKFAL VNEAQEQFPL SGLAERLGSP EAILHWQQGD
60 70 80 90 100
ERDSLVIAGA DHRLALSHLL PIVQRVAAGE LHGIGHRVVH GGEYFSGASR
110 120 130 140 150
LDAVSLQAIR QVAPLAPLHN PANLLGIEAA MKLFPTLTQV AVFDTAFHQS
160 170 180 190 200
LPEHAYRYAV PEALYREHGV RRYGFHGTSH RYVSQKAAQM AGLPADASSW
210 220 230 240 250
LVAHLGNGCS TCAVENGHSR DTSMGLTPLE GLVMGTRSGD VDPNLHSHLA
260 270 280 290 300
RTLGWSLEQI DSMLNKDSGL LGLSGLSNDM RSLEQAREQG HAGATLAIEV
310 320 330 340 350
FCYRLAKSLA AMSCALRRLD GLIFTGGIGE NSALIRSKTL EHLGLLGFAL
360 370 380 390
DPAANARCVR GVGGPIQAAH GPLVLVVPTN EERQIALDTL ALLD
Length:394
Mass (Da):42,057
Last modified:May 29, 2007 - v1
Checksum:iDE8014D8E5BD145E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA. Translation: ABP86392.1.
RefSeqiWP_003240501.1. NC_009439.1.

Genome annotation databases

EnsemblBacteriaiABP86392; ABP86392; Pmen_3644.
GeneIDi5110105.
KEGGipmy:Pmen_3644.
PATRICifig|399739.8.peg.3693.

Similar proteinsi

Entry informationi

Entry nameiACKA_PSEMY
AccessioniPrimary (citable) accession number: A4XYH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: February 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome