Reviewed, UniProtKB/Swiss-Prot A4XWX9 (PLSB_PSEMY)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Pmen_3091

Organism

Pseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]

Taxonomic identifier

399739 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049447
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A4XWX9-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 3512E74B4FA3A49D	FASTA	827	93,815
	10 20 30 40 50 60 MTRSPLRRLA FGALRRLLYL WVRSETINQS AFTLKLDRSK PVFYVLQQPS VSDLAVVDRE 70 80 90 100 110 120 CTKAGLPRPV LPVAVGEHIE PAAFFYLTPE PDWFGRQDKR GISPTLDRVV TALGQHAVDD 130 140 150 160 170 180 AQIVPVSVFW GQSPDRETSA WKLLFADSWA VTGRLRRLVS ILILGRKTRV QFSTPIHLRE 190 200 210 220 230 240 LVEQDKGQER TLRMVHRILR VHFRNQKAAV IGPDVSHRRN LVKGLVHDPL VRQAIAEEAE 250 260 270 280 290 300 REKISLEKAE AQALRYGNEI ASDYTYTVIR FLELVLSWFW NKIYDGIKVH NVEGVRDIAQ 310 320 330 340 350 360 GHEVIYVPCH RSHIDYLLLS YLLFRNGLTP PHIAAGINLN MPVIGGLLRR GGAFFMRRTF 370 380 390 400 410 420 KGNPLYTAVF NEYLHTLFSK GFPVEYFVEG GRSRTGRMLR PKTGMLAITL RSFLRSHRLP 430 440 450 460 470 480 IVFVPVYIGY ERVLEGRTYL GELRGASKKK ESIFDLFKVL GALKQRFGQV SVNFGEPIKL 490 500 510 520 530 540 AEFLDQQQPG WRQQELGPQY RPAWLNDTTN RLGERVARHL NEAASINPVN LVALALLSTS 550 560 570 580 590 600 KLALDDRALA RVLDLYLALL RAVPYSPHTT LPDGDGAALI EHVKGMDLLA EQKDALGKIL 610 620 630 640 650 660 YLDEQNAVLM TYYRNNVLHI FALPALLASF FQSSARISRE QILRFTKALY PYLQAELFIR 670 680 690 700 710 720 WEIEQLDDVV DQWLAAFVEQ GLLKVEGDVY VRPAPSSRQF VLLTLLSRSV AQTLQRFYMA 730 740 750 760 770 780 IALLLNAGQN AISAEELEDL CTVMAQRLSI LHGLNAPEFF DKSLFRHFIQ SLLDQGVLRQ 790 800 810 820 DEAGKLSHHP LLSELAEGAA KRVLPAEIRL SIRQVALDRN EDEPAAP

References

[1]

"Complete sequence of Pseudomonas mendocina ymp." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000680 Genomic DNA. Translation: ABP85845.1.
RefSeq	YP_001188577.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4XWX9.
Genome annotation databases
GeneID	5107414.
GenomeReviews	Gene locus Pmen_3091 in contig CP000680_GR.
KEGG	pmy:Pmen_3091.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEMY

Accession

Primary (citable) accession number: A4XWX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)