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A4XWU3

- GLND_PSEMY

UniProt

A4XWU3 - GLND_PSEMY

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, Pmen_3055
Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPMEN399739:GHR6-3114-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Pmen_3055
OrganismiPseudomonas mendocina (strain ymp)
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000229: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 899899Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000059224Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi399739.Pmen_3055.

Structurei

3D structure databases

ProteinModelPortaliA4XWU3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini488 – 56881HD
Add
BLAST
Domaini706 – 78479ACT 1
Add
BLAST
Domaini816 – 89782ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotation
Add
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4XWU3-1 [UniParc]FASTAAdd to Basket

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MPQMDPELFD RGQFQAELAL KSSPIAAFKK AIRHAREVLD ARFKGGRDIR    50
RLVEDRAWFV DQILQEAWKR FAWSEDADIA LLAVGGYGRG ELHPYSDIDL 100
LILLDSSDHE IFREPIEGFL TLLWDIGLEV GQSVRSVDEC AEEARADLTV 150
ITNLMESRTI AGPEHLRQRM QQVTSSEQMW PSKHFYLAKR EERKARHAKY 200
NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QFGTLNLQAL VGQGFLLESE 250
YALLSSSQEF LWKVRYALHM LAGRAEDRLL FDYQARIAAL FGYQDGDGKR 300
SIEHFMQKYY RVVMGISELS DLINQHFEEV ILRAGESGPA TPLNSRFQVR 350
DGYIEVTHPN VFKRTPFAIL EVFVLMAQNP EIKGVRADSI RLLRDSRHLI 400
DDDFRKDIRN TSLFIELFKC KEGIHRNLRR MNRYGILGRY LPEFGHIVGQ 450
MQHDLFHIYT VDAHTLNLIK HLRKFRWPEL AEKFPLASKL IDRLPKPELI 500
YLAGLYHDIG KGRGGDHSEL GAVDAEAFAR RHQLPAWDSA LIVWLVQHHL 550
VMSTTAQRKD LSDPQVIHDF AQFVGDQTHL DYLYVLTVAD INATNPSLWN 600
SWRASLLRQL YTETKRALRR GLENPLDREE QIRQTQSAAL DILVRGGTDP 650
DDAEQLWSQL GDDYFLRHTA NDVAWHTEAI LQHPADSGPL VLIKETTQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMSQLNL NIHDARIITS TSQFTLDTYV 750
VLDADGGSIG DNPARIKQIR EGLIEALKNP DEYPTIIQRR VPRQLKHFAF 800
APQVTIHNDA QRPVTILELT APDRPGLLAR IGRIFLEYDL SLQNAKIATL 850
GERVEDVFFV TDANNQPLSD PELCARLQET IVRRLSEPSA QPQSLQIDI 899
Length:899
Mass (Da):102,887
Last modified:May 29, 2007 - v1
Checksum:i6154CE68B882E4DF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000680 Genomic DNA. Translation: ABP85809.1.
RefSeqiWP_003244188.1. NC_009439.1.
YP_001188541.1. NC_009439.1.

Genome annotation databases

EnsemblBacteriaiABP85809; ABP85809; Pmen_3055.
GeneIDi5107535.
KEGGipmy:Pmen_3055.
PATRICi19912722. VBIPseMen131592_3101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000680 Genomic DNA. Translation: ABP85809.1 .
RefSeqi WP_003244188.1. NC_009439.1.
YP_001188541.1. NC_009439.1.

3D structure databases

ProteinModelPortali A4XWU3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399739.Pmen_3055.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP85809 ; ABP85809 ; Pmen_3055 .
GeneIDi 5107535.
KEGGi pmy:Pmen_3055.
PATRICi 19912722. VBIPseMen131592_3101.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PMEN399739:GHR6-3114-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ymp.

Entry informationi

Entry nameiGLND_PSEMY
AccessioniPrimary (citable) accession number: A4XWU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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