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A4XWU3

- GLND_PSEMY

UniProt

A4XWU3 - GLND_PSEMY

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciPMEN399739:GHR6-3114-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:Pmen_3055
    OrganismiPseudomonas mendocina (strain ymp)
    Taxonomic identifieri399739 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000229: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 899899Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000059224Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi399739.Pmen_3055.

    Structurei

    3D structure databases

    ProteinModelPortaliA4XWU3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini488 – 56881HDUniRule annotationAdd
    BLAST
    Domaini706 – 78479ACT 1UniRule annotationAdd
    BLAST
    Domaini816 – 89782ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 342342UridylyltransferaseAdd
    BLAST
    Regioni343 – 705363Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4XWU3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQMDPELFD RGQFQAELAL KSSPIAAFKK AIRHAREVLD ARFKGGRDIR    50
    RLVEDRAWFV DQILQEAWKR FAWSEDADIA LLAVGGYGRG ELHPYSDIDL 100
    LILLDSSDHE IFREPIEGFL TLLWDIGLEV GQSVRSVDEC AEEARADLTV 150
    ITNLMESRTI AGPEHLRQRM QQVTSSEQMW PSKHFYLAKR EERKARHAKY 200
    NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QFGTLNLQAL VGQGFLLESE 250
    YALLSSSQEF LWKVRYALHM LAGRAEDRLL FDYQARIAAL FGYQDGDGKR 300
    SIEHFMQKYY RVVMGISELS DLINQHFEEV ILRAGESGPA TPLNSRFQVR 350
    DGYIEVTHPN VFKRTPFAIL EVFVLMAQNP EIKGVRADSI RLLRDSRHLI 400
    DDDFRKDIRN TSLFIELFKC KEGIHRNLRR MNRYGILGRY LPEFGHIVGQ 450
    MQHDLFHIYT VDAHTLNLIK HLRKFRWPEL AEKFPLASKL IDRLPKPELI 500
    YLAGLYHDIG KGRGGDHSEL GAVDAEAFAR RHQLPAWDSA LIVWLVQHHL 550
    VMSTTAQRKD LSDPQVIHDF AQFVGDQTHL DYLYVLTVAD INATNPSLWN 600
    SWRASLLRQL YTETKRALRR GLENPLDREE QIRQTQSAAL DILVRGGTDP 650
    DDAEQLWSQL GDDYFLRHTA NDVAWHTEAI LQHPADSGPL VLIKETTQRE 700
    FEGGTQIFIY APDQHDFFAV TVAAMSQLNL NIHDARIITS TSQFTLDTYV 750
    VLDADGGSIG DNPARIKQIR EGLIEALKNP DEYPTIIQRR VPRQLKHFAF 800
    APQVTIHNDA QRPVTILELT APDRPGLLAR IGRIFLEYDL SLQNAKIATL 850
    GERVEDVFFV TDANNQPLSD PELCARLQET IVRRLSEPSA QPQSLQIDI 899
    Length:899
    Mass (Da):102,887
    Last modified:May 29, 2007 - v1
    Checksum:i6154CE68B882E4DF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000680 Genomic DNA. Translation: ABP85809.1.
    RefSeqiWP_003244188.1. NC_009439.1.
    YP_001188541.1. NC_009439.1.

    Genome annotation databases

    EnsemblBacteriaiABP85809; ABP85809; Pmen_3055.
    GeneIDi5107535.
    KEGGipmy:Pmen_3055.
    PATRICi19912722. VBIPseMen131592_3101.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000680 Genomic DNA. Translation: ABP85809.1 .
    RefSeqi WP_003244188.1. NC_009439.1.
    YP_001188541.1. NC_009439.1.

    3D structure databases

    ProteinModelPortali A4XWU3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399739.Pmen_3055.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP85809 ; ABP85809 ; Pmen_3055 .
    GeneIDi 5107535.
    KEGGi pmy:Pmen_3055.
    PATRICi 19912722. VBIPseMen131592_3101.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci PMEN399739:GHR6-3114-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ymp.

    Entry informationi

    Entry nameiGLND_PSEMY
    AccessioniPrimary (citable) accession number: A4XWU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3