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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPMEN399739:GHR6-3040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Pmen_2981
OrganismiPseudomonas mendocina (strain ymp)
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000229 Componenti: Chromosome

Subcellular locationi

  1. Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 834805Periplasmic nitrate reductasePRO_5000240716Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi399739.Pmen_2981.

Structurei

3D structure databases

ProteinModelPortaliA4XWM0.
SMRiA4XWM0. Positions 43-829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4XWM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSRREFAK ANAAAIAAAA AGLPLASTAS NLITEADMTR LDWNKAPCRF
60 70 80 90 100
CGTGCSVMVA TRDNRVVATH GDVKAEVNRG LNCVKGYFLS KIMYGVDRLT
110 120 130 140 150
QPLLRMKNGV YDKQGEFQPV SWDQAFDIMA QKIKQAIAEQ GPEAVGMFGS
160 170 180 190 200
GQWTVWEGYA ANKLMKAGFR SNNIDPNARH CMASAVMGFM RTFGMDEPMG
210 220 230 240 250
CYDDIEAADA FVLWGSNMAE MHPILWSRVT DRRLSHPNTR VAVLSTFEHR
260 270 280 290 300
SFDLADIPLV FKPQTDLLIL NYIANHIIES GAVNKDFVGK HTKFARGADD
310 320 330 340 350
IGYGLRADNP LEMQAKNAAK ANTWEDMSFE QFAAFVKPYT LERTAKESGV
360 370 380 390 400
AAERLKALAK LYADPKRKVM SFWTMGFNQH TRGVWANNLI YNLHLLTGKI
410 420 430 440 450
SEPGNSPFSL TGQPSACGTA REVGTFSHRL PADMLVANPK HRETAEKIWK
460 470 480 490 500
LPPGTIQEKP GFHAVEQSRK LKDGVLKVYW TQVSNNMQAG PNVMQEILPG
510 520 530 540 550
WRNPQAFVIV SDVYPTVSAQ AADLILPSAM WVEKEGAYGN AERRTQFWHQ
560 570 580 590 600
LVKAPGEAKS DLWQLVEFSK RFTTDEVWPA ELLAKAPDYK GKTLYQVLFA
610 620 630 640 650
NGQVDQFPSE QIEAGYANDE AEAFGFYLQK GLFEEYARFG RGHAHDLAPF
660 670 680 690 700
DSYHAERGLR WPVVDGKETR WRYREGHDPY VEKGSGVQFY GYPDKRALIF
710 720 730 740 750
ALPYEPPAEA PDDEFPFWLS TGRVLEHWHT GSMTQRVEEL HGAVPDALVY
760 770 780 790 800
MHPDDAKALK ARRGSEVKVI SRRGEIRARI ETRGRNKPPR GLVFVPFFDA
810 820 830
NKLINKVTLD ATDPISKQTD YKKCAVKIEL VSLA
Length:834
Mass (Da):93,453
Last modified:May 29, 2007 - v1
Checksum:i7C8384E88B6363B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA. Translation: ABP85736.1.
RefSeqiYP_001188468.1. NC_009439.1.

Genome annotation databases

EnsemblBacteriaiABP85736; ABP85736; Pmen_2981.
KEGGipmy:Pmen_2981.
PATRICi19912572. VBIPseMen131592_3026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA. Translation: ABP85736.1.
RefSeqiYP_001188468.1. NC_009439.1.

3D structure databases

ProteinModelPortaliA4XWM0.
SMRiA4XWM0. Positions 43-829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399739.Pmen_2981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP85736; ABP85736; Pmen_2981.
KEGGipmy:Pmen_2981.
PATRICi19912572. VBIPseMen131592_3026.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciPMEN399739:GHR6-3040-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ymp.

Entry informationi

Entry nameiNAPA_PSEMY
AccessioniPrimary (citable) accession number: A4XWM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: April 29, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.