Reviewed, UniProtKB/Swiss-Prot A4XWM0 (NAPA_PSEMY)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Pmen_2981

Organism

Pseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]

Taxonomic identifier

399739 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Tat-type signal Potential
	Chain	30 – 834	805	Periplasmic nitrate reductase		PRO_5000240716
Sites
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4XWM0-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 7C8384E88B6363B1	FASTA	834	93,453
	10 20 30 40 50 60 MKLSRREFAK ANAAAIAAAA AGLPLASTAS NLITEADMTR LDWNKAPCRF CGTGCSVMVA 70 80 90 100 110 120 TRDNRVVATH GDVKAEVNRG LNCVKGYFLS KIMYGVDRLT QPLLRMKNGV YDKQGEFQPV 130 140 150 160 170 180 SWDQAFDIMA QKIKQAIAEQ GPEAVGMFGS GQWTVWEGYA ANKLMKAGFR SNNIDPNARH 190 200 210 220 230 240 CMASAVMGFM RTFGMDEPMG CYDDIEAADA FVLWGSNMAE MHPILWSRVT DRRLSHPNTR 250 260 270 280 290 300 VAVLSTFEHR SFDLADIPLV FKPQTDLLIL NYIANHIIES GAVNKDFVGK HTKFARGADD 310 320 330 340 350 360 IGYGLRADNP LEMQAKNAAK ANTWEDMSFE QFAAFVKPYT LERTAKESGV AAERLKALAK 370 380 390 400 410 420 LYADPKRKVM SFWTMGFNQH TRGVWANNLI YNLHLLTGKI SEPGNSPFSL TGQPSACGTA 430 440 450 460 470 480 REVGTFSHRL PADMLVANPK HRETAEKIWK LPPGTIQEKP GFHAVEQSRK LKDGVLKVYW 490 500 510 520 530 540 TQVSNNMQAG PNVMQEILPG WRNPQAFVIV SDVYPTVSAQ AADLILPSAM WVEKEGAYGN 550 560 570 580 590 600 AERRTQFWHQ LVKAPGEAKS DLWQLVEFSK RFTTDEVWPA ELLAKAPDYK GKTLYQVLFA 610 620 630 640 650 660 NGQVDQFPSE QIEAGYANDE AEAFGFYLQK GLFEEYARFG RGHAHDLAPF DSYHAERGLR 670 680 690 700 710 720 WPVVDGKETR WRYREGHDPY VEKGSGVQFY GYPDKRALIF ALPYEPPAEA PDDEFPFWLS 730 740 750 760 770 780 TGRVLEHWHT GSMTQRVEEL HGAVPDALVY MHPDDAKALK ARRGSEVKVI SRRGEIRARI 790 800 810 820 830 ETRGRNKPPR GLVFVPFFDA NKLINKVTLD ATDPISKQTD YKKCAVKIEL VSLA

References

[1]

"Complete sequence of Pseudomonas mendocina ymp." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000680 Genomic DNA. Translation: ABP85736.1.
RefSeq	YP_001188468.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5107699.
GenomeReviews	Gene locus Pmen_2981 in contig CP000680_GR.
KEGG	pmy:Pmen_2981.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_PSEMY

Accession

Primary (citable) accession number: A4XWM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)