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A4XWM0

- NAPA_PSEMY

UniProt

A4XWM0 - NAPA_PSEMY

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Protein
Periplasmic nitrate reductase
Gene
napA, Pmen_2981
Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi55 – 551Iron-sulfur (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPMEN399739:GHR6-3040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:Pmen_2981
OrganismiPseudomonas mendocina (strain ymp)
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000229: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signal Reviewed prediction
Add
BLAST
Chaini30 – 834805Periplasmic nitrate reductaseUniRule annotation
PRO_5000240716Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi399739.Pmen_2981.

Structurei

3D structure databases

ProteinModelPortaliA4XWM0.
SMRiA4XWM0. Positions 43-829.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiQMVYNLH.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4XWM0-1 [UniParc]FASTAAdd to Basket

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MKLSRREFAK ANAAAIAAAA AGLPLASTAS NLITEADMTR LDWNKAPCRF    50
CGTGCSVMVA TRDNRVVATH GDVKAEVNRG LNCVKGYFLS KIMYGVDRLT 100
QPLLRMKNGV YDKQGEFQPV SWDQAFDIMA QKIKQAIAEQ GPEAVGMFGS 150
GQWTVWEGYA ANKLMKAGFR SNNIDPNARH CMASAVMGFM RTFGMDEPMG 200
CYDDIEAADA FVLWGSNMAE MHPILWSRVT DRRLSHPNTR VAVLSTFEHR 250
SFDLADIPLV FKPQTDLLIL NYIANHIIES GAVNKDFVGK HTKFARGADD 300
IGYGLRADNP LEMQAKNAAK ANTWEDMSFE QFAAFVKPYT LERTAKESGV 350
AAERLKALAK LYADPKRKVM SFWTMGFNQH TRGVWANNLI YNLHLLTGKI 400
SEPGNSPFSL TGQPSACGTA REVGTFSHRL PADMLVANPK HRETAEKIWK 450
LPPGTIQEKP GFHAVEQSRK LKDGVLKVYW TQVSNNMQAG PNVMQEILPG 500
WRNPQAFVIV SDVYPTVSAQ AADLILPSAM WVEKEGAYGN AERRTQFWHQ 550
LVKAPGEAKS DLWQLVEFSK RFTTDEVWPA ELLAKAPDYK GKTLYQVLFA 600
NGQVDQFPSE QIEAGYANDE AEAFGFYLQK GLFEEYARFG RGHAHDLAPF 650
DSYHAERGLR WPVVDGKETR WRYREGHDPY VEKGSGVQFY GYPDKRALIF 700
ALPYEPPAEA PDDEFPFWLS TGRVLEHWHT GSMTQRVEEL HGAVPDALVY 750
MHPDDAKALK ARRGSEVKVI SRRGEIRARI ETRGRNKPPR GLVFVPFFDA 800
NKLINKVTLD ATDPISKQTD YKKCAVKIEL VSLA 834
Length:834
Mass (Da):93,453
Last modified:May 29, 2007 - v1
Checksum:i7C8384E88B6363B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000680 Genomic DNA. Translation: ABP85736.1.
RefSeqiYP_001188468.1. NC_009439.1.

Genome annotation databases

EnsemblBacteriaiABP85736; ABP85736; Pmen_2981.
GeneIDi5107699.
KEGGipmy:Pmen_2981.
PATRICi19912572. VBIPseMen131592_3026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000680 Genomic DNA. Translation: ABP85736.1 .
RefSeqi YP_001188468.1. NC_009439.1.

3D structure databases

ProteinModelPortali A4XWM0.
SMRi A4XWM0. Positions 43-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399739.Pmen_2981.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP85736 ; ABP85736 ; Pmen_2981 .
GeneIDi 5107699.
KEGGi pmy:Pmen_2981.
PATRICi 19912572. VBIPseMen131592_3026.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi QMVYNLH.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci PMEN399739:GHR6-3040-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ymp.

Entry informationi

Entry nameiNAPA_PSEMY
AccessioniPrimary (citable) accession number: A4XWM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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