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A4XWI3 (NADK_PSEMY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:Pmen_2944
OrganismPseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]
Taxonomic identifier399739 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 295295NAD kinase HAMAP-Rule MF_00361
PRO_1000005431

Regions

Nucleotide binding72 – 732NAD By similarity
Nucleotide binding146 – 1472NAD By similarity
Nucleotide binding187 – 1926NAD By similarity

Sites

Active site721Proton acceptor By similarity
Binding site1571NAD By similarity
Binding site1741NAD By similarity
Binding site1761NAD By similarity
Binding site2471NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A4XWI3 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 2CCF0B8C7EA4BAB6

FASTA29532,267
        10         20         30         40         50         60 
MEQFRNVGII GRLGSTRVLE TVRRLKRFLI DRHLHVILED TIADVLPGHG LQTSSRKMLG 

        70         80         90        100        110        120 
EVCDLVIVVG GDGSMLGAAR ALARHKVPVL GINRGSLGFL TDIRPDELEL KVAQVLEGQY 

       130        140        150        160        170        180 
LTENRFLLEA EVRRQGEAIG QGDALNDVVL HPGKSTRMIE FELYIDGQFV CSQKADGLIV 

       190        200        210        220        230        240 
ATPTGSTAYA LSAGGPIMHP KLDAIVVVPM YPHTLSSRPI VVDGNSELKV VVSPDMTIYP 

       250        260        270        280        290 
QVSCDGQNHF TCAPGDTLHV AKKAQKLRLI HPLDHNYYEV CRTKLGWGSR LGGHD 

« Hide

References

[1]"Complete sequence of Pseudomonas mendocina ymp."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ymp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000680 Genomic DNA. Translation: ABP85699.1.
RefSeqYP_001188431.1. NC_009439.1.

3D structure databases

ProteinModelPortalA4XWI3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399739.Pmen_2944.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP85699; ABP85699; Pmen_2944.
GeneID5107662.
KEGGpmy:Pmen_2944.
PATRIC19912488. VBIPseMen131592_2987.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227221.
KOK00858.
OMATHEMLYH.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycPMEN399739:GHR6-3000-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_PSEMY
AccessionPrimary (citable) accession number: A4XWI3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: July 9, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families