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A4XVG2 (SYQ_PSEMY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:Pmen_2572
OrganismPseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]
Taxonomic identifier399739 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095502

Regions

Motif35 – 4511"HIGH" region HAMAP-Rule MF_00126
Motif269 – 2735"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2721ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4XVG2 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 5D67FA289F5940B7

FASTA55562,863
        10         20         30         40         50         60 
MSKPTVEKAA NFLRPIVQAD LDSGKHAKIV TRFPPEPNGY LHIGHAKSIC LNFGLAEEFG 

        70         80         90        100        110        120 
GQCNLRFDDT NPAKEDQEYI DAIKADVEWL GFKWAGEERY ASDYFDQLHA WAVELIKAGK 

       130        140        150        160        170        180 
AFVCDLNAEE MRTYRGSLTE PGKNSPFRDR SVEENLDLFA RMKAGEFPDG ARSLRAKIDM 

       190        200        210        220        230        240 
ASPNINLRDP ILYRIRHAHH HQTGDKWCIY PSYDFTHGQS DAIEGITHSI CTLEFEDHRP 

       250        260        270        280        290        300 
LYEWFLENLP VPAQPRQYEF ARLNLNYTIT SKRKLKQLVD EGHVQGWDDP RMSTLSGYRR 

       310        320        330        340        350        360 
RGYTPASIRA FCDMIGVNRA GGVVDIGMLE FAIREDLDAN AARAMCVLKP LKVVITNYPE 

       370        380        390        400        410        420 
GKVENLELPR HPKQDMGVRV LPFSREIYID ASDFEETPPD GFKRLIPGGE VRLRGSYVIR 

       430        440        450        460        470        480 
ADEAIKDAAG NIVELRCSYD ENTLGKNPEG RKVKGVIHWV PAAESVECEV RLYDRLFRSA 

       490        500        510        520        530        540 
NPEKSEEGGS FLDNINPDSL VVLTGCRAEP SLAQAAPEER FQFEREGYFV ADLKDSQPGK 

       550 
PVFNRTVTLR DSWGQ 

« Hide

References

[1]"Complete sequence of Pseudomonas mendocina ymp."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ymp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000680 Genomic DNA. Translation: ABP85328.1.
RefSeqYP_001188060.1. NC_009439.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399739.Pmen_2572.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP85328; ABP85328; Pmen_2572.
GeneID5108039.
KEGGpmy:Pmen_2572.
PATRIC19911688. VBIPseMen131592_2598.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycPMEN399739:GHR6-2616-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_PSEMY
AccessionPrimary (citable) accession number: A4XVG2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 29, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries