A4XTZ3 (FOLD1_PSEMY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein FolD 1

Including the following 2 domains:

1. Methylenetetrahydrofolate dehydrogenase
   EC=1.5.1.5
2. Methenyltetrahydrofolate cyclohydrolase
   EC=3.5.4.9

Gene names

Name:	folD1
Ordered Locus Names:	Pmen_2048

Organism

Pseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]

Taxonomic identifier

399739 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity. HAMAP MF_01576
Catalytic activity	5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP MF_01576 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP MF_01576
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01576
Subunit structure	Homodimer By similarity. HAMAP MF_01576
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Bifunctional protein FolD 1 HAMAP MF_01576		PRO_0000340587

Sequences

	Sequence		Length	Mass (Da)
	A4XTZ3 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 88A0F0C02DC04636	FASTA	284	30,492
	10 20 30 40 50 60 MTAQLIDGKA IAASLRQQIA QRVAERRQQG LRAPGLAVIL VGTDPASQVY VSHKRKDCEE 70 80 90 100 110 120 VGFLSRAYDL PAETSQADLL ALIDELNEDP TIDGILVQLP LPEHLDSSLL LERIRPDKDV 130 140 150 160 170 180 DGFHPYNIGR LAQRMPLLRP CTPKGIMTLL ESTGADLYGM HAVVVGASNI VGRPMAMELL 190 200 210 220 230 240 LAGCTVTVTH RFTKDLAGHV GQADIVVVAA GKPGLVKGEW IKEGAIVIDV GINRQEDGKL 250 260 270 280 VGDVVYETAL PRAGWITPVP GGVGPMTRAG LLENTLHAAE HLHK

References

[1]

"Complete sequence of Pseudomonas mendocina ymp." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ymp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000680 Genomic DNA. Translation: ABP84809.1.
RefSeq	YP_001187541.1. NC_009439.1.
3D structure databases
ProteinModelPortal	A4XTZ3.
SMR	A4XTZ3. Positions 3-283.
ModBase	Search...
Protein-protein interaction databases
STRING	A4XTZ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5109782.
GenomeReviews	Gene locus Pmen_2048 in contig CP000680_GR.
KEGG	pmy:Pmen_2048.
PATRIC	19910629. VBIPseMen131592_2077.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0190.
HOGENOM	HBG328751.
OMA	SHKRKDC.
PhylomeDB	A4XTZ3.
ProtClustDB	PRK10792.
Enzyme and pathway databases
BioCyc	PMEN399739:PMEN_2048-MONOMER.
Family and domain databases
HAMAP	MF_01576. THF_DHG_CYH. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020867. THF_DH/CycHdrlase_CS. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01491.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
PROSITE	PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD1_PSEMY

Accession

Primary (citable) accession number: A4XTZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program