Reviewed, UniProtKB/Swiss-Prot A4XTN0 (DNAE2_PSEMY)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Pmen_1934

Organism

Pseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]

Taxonomic identifier

399739 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1026 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1026	1026	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070593

Sequences

	Sequence		Length	Mass (Da)
	A4XTN0-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: CAF82455F48121EC	FASTA	1,026	115,403
	10 20 30 40 50 60 MTPDYAELHC LSNFSFQRGA SSARELFERA ARLGYRALAI TDECTLAGIV RAWQAARETG 70 80 90 100 110 120 LALIVGSEMP IEGGPKLVLL AEDLAGYQAL CRLITVARRR AEKGRYRLLR EDFDTPLAGL 130 140 150 160 170 180 LAIWLAEPQA SDSEGAWLRQ RFADRLWLGI ELHRGADDAA RLRQLQALAA RLQIPALACG 190 200 210 220 230 240 DVHMHARGRR ALQDCMTAIR QHLPVAEAGA WLFPNGERHL RRREELAELY PQELLDETLR 250 260 270 280 290 300 VAARCHFELD QLKYQYPHEL VPAGHDASSW LRHRVEEGLR WRWPDGASTK VRTQVEHELQ 310 320 330 340 350 360 LITELKYESY FLTVHDIVSW ARKQHILCQG RGSAANSTVC FALGITELNP DNSGSRLLFE 370 380 390 400 410 420 RFLSRERNEP PDIDVDFEHE RREEVIQYVF RRYGRQRAAL TAVASTYHGA GALRDVAKAL 430 440 450 460 470 480 GLPPDQVGAL ADCCGRWSDH MPDAERLREA GFDPDSPSLH RILTLAGELI GFPRHLSQHP 490 500 510 520 530 540 GGFVISEHPL QTLVPVENAS MAERTVIQWD KDDLDLVGLL KVDILALGML SALRRCFALI 550 560 570 580 590 600 ERYRGTRWTL ASLPQEDAAT YAMISRADTV GVFQIESRAQ MAMLPRLRPK TFYDLVIQVA 610 620 630 640 650 660 IVRPGPIQGD MVHPYLRRRN DEEPVEYPSD ELRPVFARTL GVPLFQEQVM ELAMVAADYS 670 680 690 700 710 720 PGEADELRRS MAAWKRHGGL EPHRQRLTSR MLEKGYTAEF TARIFRQIEG FGSYGFPESH 730 740 750 760 770 780 AASFALLTYA SCWLKCHEPA AYACALVNSW PMGFYSPDQV LQDARRHGIE VRPADVRHSA 790 800 810 820 830 840 WDCSLEPSDG EQPAIRLGLR LLRGFREEDA LRIEATRQAA PFTDVDDLCR RATLDARARE 850 860 870 880 890 900 CLADAGALRG LAGNRYRARW AVAGVEPQLP LFAGHSSREE PVALPRPTVG EDLLTDYATF 910 920 930 940 950 960 GTTLGPHPLA LLRERLRAKR CRSSHELCTV EHGRPVSVAG LVIGRQRPQT ASGVIFVTLE 970 980 990 1000 1010 1020 DEFGMVNVVV WHDLAERQRR VLLQSRLLRI DGHLESADGV RHVIAGRLTD LSPLLTGLDV KSRDFH

References

[1]

"Complete sequence of Pseudomonas mendocina ymp." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000680 Genomic DNA. Translation: ABP84696.1.
RefSeq	YP_001187428.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5110062.
GenomeReviews	Gene locus Pmen_1934 in contig CP000680_GR.
KEGG	pmy:Pmen_1934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4XTN0. MGFYSPD.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSEMY

Accession

Primary (citable) accession number: A4XTN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)