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Reviewed, UniProtKB/Swiss-Prot A4XTK4 (PDXB_PSEMY)

Last modified June 16, 2009. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Erythronate-4-phosphate dehydrogenase
    EC=1.1.1.290
Gene names
Name: pdxB
Ordered Locus Names: Pmen_1908
OrganismPseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]
Taxonomic identifier399739 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate By similarity.

Catalytic activity

4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH. HAMAP MF_01825

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5. HAMAP MF_01825

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

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Ontologies

Keywords
   Biological processPyridoxine biosynthesis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

pyridoxine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function4-phosphoerythronate dehydrogenase activity

Inferred from electronic annotation. Source: EC

NAD or NADH binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 376376Erythronate-4-phosphate dehydrogenase HAMAP MF_01825
PRO_1000070404

Regions

Nucleotide binding126 – 1272NAD By similarity
Nucleotide binding201 – 2033NAD By similarity

Sites

Active site2031 By similarity
Active site2321 By similarity
Active site2491Proton donor By similarity
Binding site451Substrate By similarity
Binding site661Substrate By similarity
Binding site1461NAD By similarity
Binding site1741NAD; via carbonyl oxygen By similarity
Binding site2271NAD By similarity
Binding site2521NAD; via amide nitrogen By similarity
Binding site2531Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
A4XTK4-1 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 1BDA2E06D974CE3C

FASTA37640,694
        10         20         30         40         50         60 
MHIVADENIP LLDEFFAAFG SIRRLPGRGI SAADVRDADL LLVRSVTQVN RALLEGSRVR 

        70         80         90        100        110        120 
FVGTCTIGTD HLDLDYFAEA GIAWSSAPGC NARGVVDYVL GSVLTLAERE GVDPAARVYG 

       130        140        150        160        170        180 
VVGAGQVGGR LVHLLRGLGW QVRVCDPPRQ AAEGGDFVSL ERIIEECDVI SLHTPLDAST 

       190        200        210        220        230        240 
RHLFDATRLA ALQPGAWLIN ASRGAVVDNA ALRTLLPQRP DLKVVLDVWE GEPQADVELA 

       250        260        270        280        290        300 
ALCQLATPHI AGYSLDGKLR GTAQIYQACC RVLGLPEQVS LDELLPAPWL SEMSIDSSAD 

       310        320        330        340        350        360 
PAWVLASLCR AVYDPRRDDA DFRRSLVGDA DARRAAFDRL RKHYPMRREI DGLRVRIQGD 

       370 
APQLAALVRA LGAATT

« Hide

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References

[1]"Complete sequence of Pseudomonas mendocina ymp."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000680 Genomic DNA. Translation: ABP84670.1.
RefSeqYP_001187402.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID5110036.
GenomeReviewsGene locus Pmen_1908 in contig CP000680_GR.
KEGGpmy:Pmen_1908.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAA4XTK4. SAPGCNA.

Family and domain databases

HAMAPMF_01825.
[Tree]
InterProIPR006139. D-isomer_2_OHA_DH.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. False negative.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePDXB_PSEMY
AccessionPrimary (citable) accession number: A4XTK4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents