Reviewed, UniProtKB/Swiss-Prot A4XT90 (ACEK_PSEMY)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Pmen_1792

Organism

Pseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]

Taxonomic identifier

399739 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000315269
Regions
	Nucleotide binding	317 – 323	7	ATP By similarity
Sites
	Active site	373	1	By similarity
	Binding site	338	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4XT90-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 5CF79D7025A93DAE	FASTA	572	66,068
	10 20 30 40 50 60 MGQQWPAGEI ARLILDGFDD YREHFRQITD GARVRFEQAQ WQEAQRASAA RINLYEEKVA 70 80 90 100 110 120 QTRERLLEGF DESLLEVGQW PLVKSAYIAL IDLRFDDELA ETWFNSIFCS LFSHDLISDG 130 140 150 160 170 180 CMFIHTTRPS LRNQPSAAQT RNYRPAGELH LALQAIFDDY RFDVSYEDLP RDLQRLEGQL 190 200 210 220 230 240 RASLPDWICK DPQQCIELFS SVLYRNKGAY LVGRIYTPDE QWPLVIPLLH REGLGIQVDA 250 260 270 280 290 300 AITDEAEVSI IFSFTRSYFM VDVAIPAEFV GFLKRILPGK HIAELYTSIG FYKHGKSEFY 310 320 330 340 350 360 RALIGHLAST DDRFIMAPGV RGMVMSVFTL PGFNTVFKII KDRFAHAKTV DRKTVIEKYR 370 380 390 400 410 420 LVKSVDRVGR MADTQEFSDF RFPKAKFEPE CLAELLEVAP STVVVEGDTV LVRHCWTERR 430 440 450 460 470 480 MTPLNLYLES ASEAQVREAL DDYGLAIKQL AAANIFPGDM LLKNFGVTRH GRVVFYDYDE 490 500 510 520 530 540 ICFLTEVNFR RIPPPRFPED EMSSEPWYSV GPMDVFPEEF PPFLFADIKQ RRLFSQLHGN 550 560 570 LYDADYWKQL QDAIRAGKVI DVFPYRRKSP VE

References

[1]

"Complete sequence of Pseudomonas mendocina ymp." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000680 Genomic DNA. Translation: ABP84556.1.
RefSeq	YP_001187288.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4XT90.
Genome annotation databases
GeneID	5109372.
GenomeReviews	Gene locus Pmen_1792 in contig CP000680_GR.
KEGG	pmy:Pmen_1792.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEMY

Accession

Primary (citable) accession number: A4XT90

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)