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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Pseudomonas mendocina (strain ymp)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATPUniRule annotation1
Active sitei308Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 287ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMEN399739:G1G8Q-1213-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Pmen_1191
OrganismiPseudomonas mendocina (strain ymp)
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000229 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000121141 – 341RNA 3'-terminal phosphate cyclaseAdd BLAST341

Interactioni

Protein-protein interaction databases

STRINGi399739.Pmen_1191

Structurei

3D structure databases

ProteinModelPortaliA4XRJ0
SMRiA4XRJ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKX Bacteria
COG0430 LUCA
HOGENOMiHOG000015264
KOiK01974
OMAiANRPKPG
OrthoDBiPOG091H0UB7

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 2 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

A4XRJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKDFIELDG AIGGGQILRS ALSLSMLTGK PFRIHNIRAK RSRPGLLRQH
60 70 80 90 100
LTAVTAAAQI SSADAEGTQI GSQSLSFAPG AIRGGDYEFA IGTAGSCSLV
110 120 130 140 150
VQTLLPALLH ADQPSRVRIS GGTHNPLAPP FEFLERAWLP LLKRMGAQVE
160 170 180 190 200
IELLRHGFAP AGGGALVMQV TPSALRPLHL ETPGPIHSQQ ATALVADVPG
210 220 230 240 250
HVAERELARV GKRLKWPEQA LKGIWLDKQI GPGNVLLLEI ACGEVTELFS
260 270 280 290 300
SIGQSGVRAE RVADQAVDQA RQWLHSGAAV DEHLADQLLL PLAMAGGGSF
310 320 330 340
STTHMSEHLS SNIQVIRQFL NVEIVCTQAN ERILRVELRQ G
Length:341
Mass (Da):36,513
Last modified:May 29, 2007 - v1
Checksum:i6AD97C7AC97E99EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA Translation: ABP83956.1
RefSeqiWP_012017846.1, NC_009439.1

Genome annotation databases

EnsemblBacteriaiABP83956; ABP83956; Pmen_1191
GeneIDi5108692
KEGGipmy:Pmen_1191
PATRICifig|399739.8.peg.1203

Similar proteinsi

Entry informationi

Entry nameiRTCA_PSEMY
AccessioniPrimary (citable) accession number: A4XRJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: March 28, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health