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Protein

Alginate lyase

Gene

algL

Organism
Pseudomonas mendocina (strain ymp)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. May serve to degrade mislocalized alginate that is trapped in the periplasmic space.UniRule annotation

Catalytic activityi

Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei249SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported

Enzyme and pathway databases

BioCyciPMEN399739:GHR6-1030-MONOMER.

Protein family/group databases

CAZyiPL5. Polysaccharide Lyase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Alginate lyaseUniRule annotation (EC:4.2.2.3UniRule annotation)
Alternative name(s):
Poly(beta-D-mannuronate) lyaseUniRule annotation
Gene namesi
Name:algLUniRule annotation
Ordered Locus Names:Pmen_1024Imported
OrganismiPseudomonas mendocina (strain ymp)Imported
Taxonomic identifieri399739 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000229 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

PeriplasmUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotationAdd BLAST21
ChainiPRO_500900687122 – 358Alginate lyaseUniRule annotationAdd BLAST337

Interactioni

Protein-protein interaction databases

STRINGi399739.Pmen_1024.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 296Alginate_lyaseInterPro annotationAdd BLAST242

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 59Substrate bindingUniRule annotation2
Regioni131 – 132Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the polysaccharide lyase 5 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiENOG4105FN4. Bacteria.
ENOG4111N6J. LUCA.
HOGENOMiHOG000247392.
KOiK01729.
OMAiAAWSVMA.
OrthoDBiPOG091H0I15.

Family and domain databases

HAMAPiMF_00557. Alginate_lyase. 1 hit.
InterProiView protein in InterPro
IPR022859. Alginate_lyase.
IPR008397. Alginate_lyase_dom.
IPR008929. Chondroitin_lyas.
PfamiView protein in Pfam
PF05426. Alginate_lyase. 1 hit.
SUPFAMiSSF48230. SSF48230. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4XR26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRRLAPLTL LAVLLSAPAF AELQPPAGYH APVGQRDGKA PACSAPPQPY
60 70 80 90 100
TGELQFTSKY AGSDSARATL NRNAEKNFRK QTEGITRLEK EAGRLITGYM
110 120 130 140 150
RTGQRERLEC AIEWLDHWAS ADALESEQYN HTGKSMRKWA LGSLAGAWLR
160 170 180 190 200
LKFSESQPLA DYPQQSERIE AWFVRLAEHT VREWSDLPLK KINNHSYWAA
210 220 230 240 250
WSVMAVAVIG DRRDLFDWSV GQFRIAAGQV DDEGFLDNEM RRRQRALAYH
260 270 280 290 300
NYALPPLAMI AAFAQANGVD LRGENHGALQ RLAERVLQGA RDPGAFAQRT
310 320 330 340 350
GSRQDMEELR KKFKYAWLAP YCTLYVCNEQ TRELNQEMGP FNSFRLGGEV

TQVFGGGG
Length:358
Mass (Da):40,264
Last modified:May 29, 2007 - v1
Checksum:i882C958D269D52B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000680 Genomic DNA. Translation: ABP83792.1.
RefSeqiWP_011921100.1. NC_009439.1.

Genome annotation databases

EnsemblBacteriaiABP83792; ABP83792; Pmen_1024.
GeneIDi5108646.
KEGGipmy:Pmen_1024.
PATRICifig|399739.8.peg.1033.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA4XR26_PSEMY
AccessioniPrimary (citable) accession number: A4XR26
Entry historyiIntegrated into UniProtKB/TrEMBL: May 29, 2007
Last sequence update: May 29, 2007
Last modified: June 7, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported