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A4XQS6 (DDL_PSEMY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Pmen_0924
OrganismPseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]
Taxonomic identifier399739 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341155

Regions

Domain111 – 306196ATP-grasp
Nucleotide binding137 – 19256ATP By similarity

Sites

Metal binding2601Magnesium or manganese 1 By similarity
Metal binding2731Magnesium or manganese 1 By similarity
Metal binding2731Magnesium or manganese 2 By similarity
Metal binding2751Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4XQS6 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: A047906A8C20E8D5

FASTA31333,179
        10         20         30         40         50         60 
MSLHSSLDPK AFGRVAVLFG GKSAEREVSL KSGNAVLSAL QAAGVDAFGI DVGDDFLQRL 

        70         80         90        100        110        120 
ASEKIDRAFI VLHGRGGEDG SMQGLLECAG IPYTGSGILA SALAMDKLRT KQVWHSLGLP 

       130        140        150        160        170        180 
TPRHAVLTSQ ADCEAAAAEL GFPLIVKPAH EGSSIGMAKV ESVEALIAAW QDAARYDSQV 

       190        200        210        220        230        240 
LVEQWIAGPE YTVAVLRGEV LPPIGLGTPH SFYDYDAKYL ADDTQYRIPC GLSAEKEAEL 

       250        260        270        280        290        300 
KELTARACEA VGTQGWARAD VMQDASGQFW LLEVNTVPGM TDHSLVPMAA RAAGLDFQQL 

       310 
VLAILADSVE ARG 

« Hide

References

[1]"Complete sequence of Pseudomonas mendocina ymp."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ymp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000680 Genomic DNA. Translation: ABP83692.1.
RefSeqYP_001186424.1. NC_009439.1.

3D structure databases

ProteinModelPortalA4XQS6.
SMRA4XQS6. Positions 13-306.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399739.Pmen_0924.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP83692; ABP83692; Pmen_0924.
GeneID5108782.
KEGGpmy:Pmen_0924.
PATRIC19908280. VBIPseMen131592_0933.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycPMEN399739:GHR6-929-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PSEMY
AccessionPrimary (citable) accession number: A4XQS6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways