A4XQA3 (RLMG_PSEMY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Pmen_0751

Organism

Pseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]

Taxonomic identifier

399739 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence ABP83519.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366478

Sequences

	Sequence		Length	Mass (Da)
	A4XQA3 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: EAC2DE5495969F09	FASTA	374	40,592
	10 20 30 40 50 60 MPIFTTPFAS LELLRQPHQP NEPLQAFDAA DEYLLNHLHE QGLRAEDSLL LLNDSFGALA 70 80 90 100 110 120 CSLAGRCQVT SSSDSHLGFI ALENNLAGNG LNRDAVRFLP SSETPQGPFD WVLIRVPKTL 130 140 150 160 170 180 ALLEEQLIRL HGQLAPGARV VAAAMVKHLP RAAGDLLEKY IGPVQASLAV KKARLLLATP 190 200 210 220 230 240 EAKAAPHSPY PTRYRLDKPA LELINHANVF CRDGLDIGTR AFLPHLPRHL DARRVADLGC 250 260 270 280 290 300 GNGVLGIAYA LGSPQAQLTL VDESYMAVQS ARENWAAALG ERPATIRAGD GLAEQPAGSL 310 320 330 340 350 360 DLVLCNPPFH QQQVVGDFLA WRMFQQARAA LVTGGELWIV GNRHLGYHAK LARLFRGVEQ 370 VAANPKFVVL KASK

References

[1]

"Complete sequence of Pseudomonas mendocina ymp." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ymp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000680 Genomic DNA. Translation: ABP83519.1. Different initiation.
RefSeq	YP_001186251.1. NC_009439.1.
3D structure databases
ProteinModelPortal	A4XQA3.
ModBase	Search...
Protein-protein interaction databases
STRING	A4XQA3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5108845.
GenomeReviews	Gene locus Pmen_0751 in contig CP000680_GR.
KEGG	pmy:Pmen_0751.
PATRIC	19907932. VBIPseMen131592_0762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
ProtClustDB	CLSK868782.
Enzyme and pathway databases
BioCyc	PMEN399739:PMEN_0751-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEMY

Accession

Primary (citable) accession number: A4XQA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program