Reviewed, UniProtKB/Swiss-Prot A4XPI5 (BETA_PSEMY)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Pmen_0481

Organism

Pseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]

Taxonomic identifier

399739 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Choline dehydrogenase		PRO_1000046569
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4XPI5-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: AAFF3254370B986D	FASTA	565	62,252
	10 20 30 40 50 60 MSHEFDYIII GAGSAGNVLA TRLTEDADVS VLLLEAGGPD YRLDFRTQMP AALAFPLQGR 70 80 90 100 110 120 RYNWAYETDP EPHMNNRRME CGRGKGLGGS SLINGMCYIR GNALDFDNWA KAKGLEDWTY 130 140 150 160 170 180 HDCLPYFRKA ESRDIGPNDY HGGDGPVSVT TPKAGNNPLF HAMVEAGVQA GYPRTDDLNG 190 200 210 220 230 240 YQQEGFGPMD RTVTPQGRRA STARGYLDQA RERPNLTIVT HATTDRILFD GKRASGVSYL 250 260 270 280 290 300 IGNANDATEA RARREVLLCA GAIASPQILQ RSGVGPAALL RELDIALVHE LPGVGQNLQD 310 320 330 340 350 360 HLEMYLQYAC TQPVSLYPAL KLLNQPGIGA QWLFTGNGIG ASNQFEAGGF IRTRPEFAWP 370 380 390 400 410 420 NIQFHFLPVA INYNGSNAVN EHGFQAHVGS MRSPSRGRIQ LKSKDPRQHP SILFNYMSHE 430 440 450 460 470 480 QDWQEFRDAI RITREIMAQP ALDSYRGREI SPGIDCQSDA ELDAFIREHA ETAFHPSCSC 490 500 510 520 530 540 KMGEDDMAVV DGQGRVHGVQ GLRVVDASIM PEIITGNLNA TTIMMAEKIA DRIRDRQPLP 550 560 RSNAPYFVAG ERPVRGQPQR AVSAA

References

[1]

"Complete sequence of Pseudomonas mendocina ymp." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000680 Genomic DNA. Translation: ABP83251.1.
RefSeq	YP_001185983.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5108400.
GenomeReviews	Gene locus Pmen_0481 in contig CP000680_GR.
KEGG	pmy:Pmen_0481.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_PSEMY

Accession

Primary (citable) accession number: A4XPI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)